Anti-SLO antibodies specifically react with SLO antigens precoated on latex beads to form insoluble complexes, resulting in turbidity increasing, and then the increasing of absorbance is detected by automatic biochemical analyzer. The calibration curve was fitted according to the relationship between absorbance values and ASO (Anti-SLO Antibodies) concentrations (Fig.1).
Fig.1 Calibration curve for ASO in latex-enhanced immunoturbidimetric assay (LETIA)
The high-value ASO serum was 2-fold serially diluted with physiological saline, and measured on the LETIA platform. Fig.2 showed that the measured ASO concentrations were gradual declined along with the serial dilution of blood samples.
Fig.2 Determination of the ASO concentration by serial dilution of donor serum.
Two levels of ASO controls were analyzed in replicates of three using MBS1491645 SLO protein on the LETIA platform. Data from this study are summarized in the following table, the relative bias was <2%.
A two-member buffered protein-based panel was assayed, using a single lot of reagents, in replicates of twenty at two separate times on the LETIA platform. As shown in the Table, the system showed excellent precision with CV <=2%.
SLO protein was also with random blood samples from donor (n=100). Fig.3 showed that the correlation coefficient(r) is as high as 0.99 between latex reagents and another commercial ASO immunoassay. These results show good agreement between the two systems.
Fig.3 Comparison of ASO immunoassay and another commercial MyBioSource assays
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