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SHISA5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1491697

Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis. More details

$230 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SHISA5, Polyclonal Antibody
Full Product Name
SHISA5 Antibody
Product Gene Name
Product Synonym Names
Protein shisa-5; SHISA5; SCOTIN; PSEC0133; Putative NF-kappa-B-activating protein 120; Scotin
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%, Protein G Purified
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Matching Pairs
Unconjugated Antibody: SHISA5 (MBS1491697)
Biotin Conjugated Antibody: SHISA5 (MBS1493301)
Matching Pairs
Unconjugated Antibody: SHISA5 (MBS1491697)
HRP Conjugated Antibody: SHISA5 (MBS1494607)
Molecular Weight
24,855 Da
OMIM
607290
3D Structure
ModBase 3D Structure for Q8N114
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Protein shisa-5 protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-366145
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-SHISA5 antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-SHISA5 antibody
Recommended dilution: IHC:1:20-1:200

Immunohistochemistry (IHC) of anti-SHISA5 antibody

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS1491697 at dilution of 1:100
Rabbit Anti-SHISA5 Polyclonal Antibody – Cat# MBS1491697

Immunofluorescence (IF) of anti-SHISA5 antibody

Immunofluorescence staining of A549 cells with MBS1491697 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Rabbit Anti-SHISA5 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1491697

Immunoprecipitation (IP) of anti-SHISA5 antibody

Immunoprecipitating SHISA5 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG (1ug)instead of MBS1491697 in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS1491697 (6ug)+ HepG2 whole cell lysate (500ug)
Lane 3: HepG2 whole cell lysate (10ug)
Rabbit Anti-SHISA5 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS1491697

Other Notes

Small volumes of anti-SHISA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SHISA5 purchase

MBS1491697 is an antibody targeting SHISA5. Also known as SHISA5 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SHISA5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein shisa-5 isoform b
NCBI Official Synonym Full Names
shisa family member 5
NCBI Official Symbol
SHISA5
NCBI Official Synonym Symbols
SCOTIN
UniProt Protein Name
Protein shisa-5
UniProt Synonym Protein Names
Putative NF-kappa-B-activating protein 120; Scotin
Protein Family
UniProt Gene Name
SHISA5
UniProt Synonym Gene Names
SCOTIN
UniProt Entry Name
SHSA5_HUMAN
NCBI Protein Information
protein shisa-5

NCBI Summary for SHISA5

This gene encodes a member of the shisa family. The encoded protein is localized to the endoplasmic reticulum, and together with p53 induces apoptosis in a caspase-dependent manner. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

UniProt Comments for SHISA5

SCOTIN: Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis. Belongs to the shisa family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: endoplasmic reticulum membrane; integral to membrane; nuclear membrane

Molecular Function: protein binding; signal transducer activity; WW domain binding

Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SHISA5 antibody

 Reference Product  PubMed Publications
 TP53 antibody  >2 publications with SHISA5 and TP53

Pathways associated with anti-SHISA5 antibody

Organs/Tissues associated with anti-SHISA5 antibody

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