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DNTT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1494380

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig… More details

$230 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNTT, Polyclonal Antibody
Full Product Name
DNTT Antibody
Product Gene Name
Product Synonym Names
DNA nucleotidylexotransferase (EC:2.7.7.313 Publications; DNTT; TDT1; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%, Protein G Purified
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Matching Pairs
Unconjugated Antibody: DNTT (MBS1494380)
HRP Conjugated Antibody: DNTT (MBS1493725)
Matching Pairs
Unconjugated Antibody: DNTT (MBS1494380)
FITC Conjugated Antibody: DNTT (MBS1494392)
Molecular Weight
58,408 Da
OMIM
187410
3D Structure
ModBase 3D Structure for P04053
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human DNA nucleotidylexotransferase protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-19441
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DNTT antibody
ELISA (EIA), Immunohistochemistry (IHC)

Immunohistochemistry (IHC) of anti-DNTT antibody

Immunohistochemistry of paraffin-embedded human thymus tissue using MBS1494380 at dilution of 1:100
Rabbit Anti-DNTT Polyclonal Antibody – Cat# MBS1494380

Immunofluorescence (IF) of anti-DNTT antibody

Immunofluorescent analysis of PC3 cells using MBS1494380 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Rabbit Anti-DNTT Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1494380

Other Notes

Small volumes of anti-DNTT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNTT purchase

MBS1494380 is an antibody targeting DNTT. Also known as DNTT Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DNTT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA nucleotidylexotransferase isoform 2
NCBI Official Synonym Full Names
DNA nucleotidylexotransferase
NCBI Official Symbol
DNTT
NCBI Official Synonym Symbols
TDT
UniProt Protein Name
DNA nucleotidylexotransferase
UniProt Synonym Protein Names
Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase
UniProt Gene Name
DNTT
UniProt Synonym Gene Names
; Terminal transferase
UniProt Entry Name
TDT_HUMAN
NCBI Protein Information
DNA nucleotidylexotransferase

NCBI Summary for DNTT

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for DNTT

DNTT: Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. Belongs to the DNA polymerase type-X family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.7.31; Transferase

Chromosomal Location of Human Ortholog: 10q23-q24

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: DNA binding; DNA nucleotidylexotransferase activity; DNA-directed DNA polymerase activity; metal ion binding; protein binding

Biological Process: DNA metabolic process; DNA modification
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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