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Malate Dehydrogenase (MDH1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1495197

MDH1(Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It… More details

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Product Overview

Online Datasheet in PDF
Product Name
Malate dehydrogenase (MDH1), Polyclonal Antibody
Full Product Name
Rabbit anti-human Malate dehydrogenase, cytoplasmic polyclonal Antibody(MDH1)
Product Gene Name
anti-MDH1 antibody
Product Synonym Names
Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase; MDH1; MDHA
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Antigen Affinity Purified
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Molecular Weight
38,628 Da
OMIM
154200
3D Structure
ModBase 3D Structure for P40925
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Malate dehydrogenase, cytoplasmic protein (1-334aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49233 / sc-99013
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MDH1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-MDH1 antibody
Recommended dilution: WB:1:500-5000
IHC:1:20-1:200

Western Blot (WB) of anti-MDH1 antibody

All lanes: Malate dehydrogenase, cytoplasmic antibody at 4ug/ml
Lane 1:HepG2 whole cell lysate
Lane 2:Hela whole cell lysate
Lane 3:mouse kidney tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 37,28,39 kDa
Observed band size: 36 kDa
Rabbit Anti-MDH1 Polyclonal Antibody – Cat# MBS1495197

Immunohistochemistry (IHC) of anti-MDH1 antibody

Immunohistochemistry of paraffin-embedded human skin tissue using MBS1495197 at dilution 1:100
Rabbit Anti-MDH1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1495197

Immunoprecipitation (IP) of anti-MDH1 antibody

Immunoprecipitating MDH1 in HepG2 whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of MBS1495197 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2:MBS1495197(4ug)+ HepG2 whole cell lysate(500ug)
Lane 3: HepG2 whole cell lysate (20ug)
Rabbit Anti-MDH1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS1495197

Other Notes

Small volumes of anti-MDH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MDH1 purchase

MBS1495197 is an antibody targeting MDH1. Also known as Rabbit anti-human Malate dehydrogenase, cytoplasmic polyclonal Antibody(MDH1). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MDH1. It may not necessarily be applicable to this product.

NCBI GI #
312283701
NCBI GeneID
4190
NCBI Accession #
NP_001186040.1
UniProt Primary Accession #
P40925
UniProt Secondary Accession #
Q6I9V0; B2R5V5; B4DUN2; B7Z3I7; F5H098
UniProt Related Accession #
P40925
GenBank Nucleotide #
NM_001199111.1
NCBI Official Full Name
malate dehydrogenase, cytoplasmic isoform 2
NCBI Official Synonym Full Names
malate dehydrogenase 1
NCBI Official Symbol
MDH1
NCBI Official Synonym Symbols
MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375
UniProt Protein Name
Malate dehydrogenase, cytoplasmic
UniProt Synonym Protein Names
Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase (EC:1.1.1.96)
Protein Family
Malate dehydrogenase
UniProt Gene Name
MDH1
UniProt Synonym Gene Names
MDHA
UniProt Entry Name
MDHC_HUMAN
NCBI Protein Information
malate dehydrogenase, cytoplasmic

NCBI Summary for MDH1

This gene encodes an enzyme that catalyzes the reversible oxidation of malate to oxaloacetate using the NAD/NADH cofactor system in the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one found in the mitochondrial matrix and the other in the cytoplasm. This protein represents the cytosolic isozyme, which has a pivotal role in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. A recent study showed that two protein isoforms are produced from this mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism, and that these C-terminally distinct isoforms have different subcellular localization. Alternatively spliced transcript variants have also been found for this gene. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Oct 2015]
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UniProt Comments for MDH1

MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; Carbohydrate Metabolism - pyruvate; EC 1.1.1.37; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.1.96

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: centrosome; cytoplasm; cytosol; extracellular space; mitochondrion; myelin sheath

Molecular Function: diiodophenylpyruvate reductase activity; L-malate dehydrogenase activity; malic enzyme activity; NAD binding; protein binding

Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process; malate metabolic process; NADH metabolic process; oxaloacetate metabolic process; positive regulation of defense response to virus by host; tricarboxylic acid cycle
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Research Articles on MDH1

1. adipogenic differentiation may be regulated by the acetylation of MDH1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MDH1 antibody

 Reference Product  PubMed Publications
 MDH2 antibody  >37 publications with MDH1 and MDH2
 PGM1 antibody  >13 publications with MDH1 and PGM1
 ME1 antibody  >6 publications with MDH1 and ME1
 GOT1 antibody  >5 publications with MDH1 and GOT1
 GOT2 antibody  >3 publications with MDH1 and GOT2
 ACLY antibody  >2 publications with MDH1 and ACLY

Pathways associated with anti-MDH1 antibody

 Products by Pathway  Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
 Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway antibodies  Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway Diagram
 Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway antibodies  Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram

Diseases associated with anti-MDH1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >6 publications with MDH1 and Nervous System Diseases
 Brain Diseases Antibodies  >4 publications with MDH1 and Brain Diseases
 Disease Models, Animal Antibodies  >3 publications with MDH1 and Disease Models, Animal
 Heart Diseases Antibodies  >2 publications with MDH1 and Heart Diseases
 Vascular Diseases Antibodies  >2 publications with MDH1 and Vascular Diseases
 Neurobehavioral Manifestations Antibodies  >1 publications with MDH1 and Neurobehavioral Manifestations
 Seizures Antibodies  >1 publications with MDH1 and Seizures
 Prenatal Injuries Antibodies  >1 publications with MDH1 and Prenatal Injuries
 Fibrosis Antibodies  >1 publications with MDH1 and Fibrosis
 Kidney Diseases Antibodies  >1 publications with MDH1 and Kidney Diseases

Organs/Tissues associated with anti-MDH1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >15 publications with MDH1 and Blood
 Brain Antibodies  >10 publications with MDH1 and Brain
 Muscle Antibodies  >7 publications with MDH1 and Muscle
 Liver Antibodies  >5 publications with MDH1 and Liver
 Heart Antibodies  >4 publications with MDH1 and Heart
 Kidney Antibodies  >3 publications with MDH1 and Kidney
 Lung Antibodies  >3 publications with MDH1 and Lung
 Nerve Antibodies  >3 publications with MDH1 and Nerve
 Intestine Antibodies  >3 publications with MDH1 and Intestine
 Thyroid Antibodies  >2 publications with MDH1 and Thyroid
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