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Serine/Threonine-Protein Kinase Nek2 (NEK2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1497174

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and… More details

$230 / 0.05 mL +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Serine/threonine-protein kinase Nek2, Polyclonal Antibody
Full Product Name
Rabbit anti-human Serine/threonine-protein kinase Nek2 polyclonal Antibody(NEK2)
Product Gene Name
anti-NEK2 antibody
Product Synonym Names
HSPK 21; Never in mitosis A-related kinase 2; NimA-related protein kinase 2; NimA-like protein kinase 1; NEK2; NEK2A; NLK1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Antigen Affinity Purified
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Molecular Weight
50,909 Da
OMIM
604043
3D Structure
ModBase 3D Structure for P51955
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Serine/threonine-protein kinase Nek2 protein (1-135aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7439 / sc-7440 / sc-33167
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-NEK2 antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-NEK2 antibody
Recommended dilution: IHC:1:20-1:200

Immunohistochemistry (IHC) of anti-NEK2 antibody

Immunohistochemistry of paraffin-embedded human kidney using MBS1497174 at dilution 1:100
Rabbit Anti-NEK2 Polyclonal Antibody – Cat# MBS1497174

Other Notes

Small volumes of anti-NEK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEK2 purchase

MBS1497174 is an antibody targeting NEK2. Also known as Rabbit anti-human Serine/threonine-protein kinase Nek2 polyclonal Antibody(NEK2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NEK2. It may not necessarily be applicable to this product.

NCBI GI #
323510688
NCBI GeneID
4751
NCBI Accession #
NP_001191111.1
UniProt Primary Accession #
P51955
UniProt Secondary Accession #
Q53FD6; Q5I1Z9; Q5VXZ1; Q6NZX8; Q7Z634; Q86XH2; Q96QN9
UniProt Related Accession #
P51955
GenBank Nucleotide #
NM_001204182.1
NCBI Official Full Name
serine/threonine-protein kinase Nek2 isoform 3
NCBI Official Synonym Full Names
NIMA related kinase 2
NCBI Official Symbol
NEK2
NCBI Official Synonym Symbols
NLK1; RP67; NEK2A; HsPK21; PPP1R111
UniProt Protein Name
Serine/threonine-protein kinase Nek2
UniProt Synonym Protein Names
HSPK 21; Never in mitosis A-related kinase 2; NimA-related protein kinase 2; NimA-like protein kinase 1
Protein Family
Probable serine/threonine-protein kinase
UniProt Gene Name
NEK2
UniProt Synonym Gene Names
NEK2A; NLK1; NimA-related protein kinase 2
UniProt Entry Name
NEK2_HUMAN
NCBI Protein Information
serine/threonine-protein kinase Nek2

NCBI Summary for NEK2

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
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UniProt Comments for NEK2

NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation

Protein type: Nucleolus; EC 2.7.11.1; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: centrosome; condensed nuclear chromosome; cytoplasm; cytosol; kinetochore; microtubule; midbody; nucleolus; nucleus; protein complex; spindle pole

Molecular Function: ATP binding; metal ion binding; protein binding; protein kinase activity; protein phosphatase binding; protein serine/threonine kinase activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; blastocyst development; cell division; centrosome separation; chromosome segregation; G2/M transition of mitotic cell cycle; meiotic cell cycle; mitosis; mitotic cell cycle; mitotic sister chromatid segregation; negative regulation of DNA binding; organelle organization and biogenesis; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of attachment of spindle microtubules to kinetochore; regulation of mitosis; regulation of mitotic centrosome separation; spindle assembly

Disease: Retinitis Pigmentosa 67
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Research Articles on NEK2

1. Nek2A is degraded in mitosis regardless of enforced spindle checkpoint activation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEK2 antibody

 Reference Product  PubMed Publications
 NDC80 antibody  >9 publications with NEK2 and NDC80
 CDC20 antibody  >8 publications with NEK2 and CDC20
 CEP250 antibody  >8 publications with NEK2 and CEP250
 MAD2L1 antibody  >3 publications with NEK2 and MAD2L1
 CDC27 antibody  >1 publications with NEK2 and CDC27

Pathways associated with anti-NEK2 antibody

 Products by Pathway  Pathway Diagram
 APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies  APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Anchoring Of The Basal Body To The Plasma Membrane Pathway antibodies  Anchoring Of The Basal Body To The Plasma Membrane Pathway Diagram
 Assembly Of The Primary Cilium Pathway antibodies  Assembly Of The Primary Cilium Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram

Diseases associated with anti-NEK2 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >29 publications with NEK2 and Breast Neoplasms
 Carcinoma Antibodies  >29 publications with NEK2 and Carcinoma
 Adenocarcinoma Antibodies  >17 publications with NEK2 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >11 publications with NEK2 and Cell Transformation, Neoplastic
 Liver Neoplasms Antibodies  >8 publications with NEK2 and Liver Neoplasms
 Lung Neoplasms Antibodies  >8 publications with NEK2 and Lung Neoplasms
 Neoplasm Metastasis Antibodies  >8 publications with NEK2 and Neoplasm Metastasis
 Lung Diseases Antibodies  >7 publications with NEK2 and Lung Diseases
 Carcinoma, Hepatocellular Antibodies  >6 publications with NEK2 and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >5 publications with NEK2 and Neoplasms, Experimental

Organs/Tissues associated with anti-NEK2 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >11 publications with NEK2 and Lung
 Liver Antibodies  >11 publications with NEK2 and Liver
 Lymph Node Antibodies  >8 publications with NEK2 and Lymph Node
 Kidney Antibodies  >8 publications with NEK2 and Kidney
 Testis Antibodies  >8 publications with NEK2 and Testis
 Blood Antibodies  >7 publications with NEK2 and Blood
 Ovary Antibodies  >6 publications with NEK2 and Ovary
 Prostate Antibodies  >5 publications with NEK2 and Prostate
 Bone Antibodies  >5 publications with NEK2 and Bone
 Brain Antibodies  >4 publications with NEK2 and Brain
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