All lanes: SLAM family member 7 antibody at 3ug/ml
Lane 1:Jurkat whole cell lysate
Lane 2:K562 whole cell lysate
Lane 3:mouse spleen tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 38,26,18,33,23,22 kDa
Observed band size: 37 kDa
Immunohistochemistry of paraffin-embedded human epityphlon using MBS1497455 at dilution 1:100
(MBS1497455) diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
(MBS1497455) diluted at 1:200 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB
Immunofluorescence staining of U87 cell with (MBS1497455) at 1:50, counter- stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Positive WB detected in: U87 whole cell
lysate,A549 whole cell lysate,U251 whole cell lysate
All lanes: SLAMF7 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25-80 kDa
Observed band size: 55/80 kDa