E3 SUMO-Protein Ligase PIAS1 (PIAS1) Antibody – Rabbit Polyclonal

This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. [provided by RefSeq, Jul 2008]

PIAS1: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Interacts with NCOA2 and AR. Interacts with NR2C1; the interaction promotes its sumoylation. Interacts with DDX21, CSRP2, AXIN1, JUN, UBE2I, SUMO1, SATB2, PLAG1, TP53 and STAT1 (dimer), following IFNA1-stimulation. Interacts with SP3 (preferentially when SUMO-modified). Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with STAT1. Interacts with CHUK/IKKA; this interaction induces PIAS1 phosphorylation. Interacts with PTK2/FAK1; the interaction promotes its sumoylation. Interacts with DDX5. Expressed in numerous tissues with highest level in testis. Belongs to the PIAS family.

Protein type: Transcription, coactivator/corepressor; EC 6.3.2.-; Nuclear receptor co-regulator; SUMO conjugating system

Chromosomal Location of Human Ortholog: 15q

Cellular Component: nuclear speck; nucleoplasm; nucleus; PML body

Molecular Function: androgen receptor binding; DNA binding; enzyme binding; ligase activity; protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor binding; ubiquitin protein ligase binding; zinc ion binding

Biological Process: androgen receptor signaling pathway; cellular protein metabolic process; cytokine and chemokine mediated signaling pathway; fat cell differentiation; JAK-STAT cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein sumoylation; positive regulation of transcription, DNA-dependent; post-translational protein modification; protein sumoylation; regulation of cell proliferation; transcription, DNA-dependent