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MYC Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1497694

Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein. It can help to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. Myc Tag also can be used to isolate… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MYC, Monoclonal Antibody
Full Product Name
Mouse Anti-MYC monoclonal Antibody
Product Gene Name
Product Synonym Names
bHLHe39; c Myc; MRTL; MYC; Myc proto oncogene protein; MYC tag; Proto oncogene c Myc; Transcription factor p64
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5F92F9
Host
Mouse
Form/Format
Liquid
Purity/Purification
>95%, Protein G Purified
Preparation and Storage
Upon receipt store at -20 degree C or -80 degree C. Avoid repeated freeze.
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
EQKLISEEDL (Myc) synthetic peptide conjugate to KLH

Product Categories/Family for anti-MYC antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MYC antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes for anti-MYC antibody
WB:1:500-1:5000
IF:1:100-1:300
IP:1:1000-1:1500

Western Blot (WB) of anti-MYC antibody

WB:Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6ug/ml
Lane 1:Recombinant Myc-tagged fusion Protein at 50ng
Lane 2:Recombinant Myc-tagged fusion Protein at 25ng
Lane 3:Recombinant Myc-tagged fusion Protein at 12.5ng
Lane 4:Recombinant Myc-tagged fusion Protein at 6.25ng
Lane 5:Recombinant Myc-tagged fusion Protein at 3.125ng
Lane 6:Recombinant Myc-tagged fusion Protein at 1.5625ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 50kd
Observed band size: 50kd
Mouse Anti-MYC Monoclonal Antibody – Cat# MBS1497694

Immunofluorescence (IF) of anti-MYC antibody

Immunofluorescence staining of transfected HEK293 cells with MBS1497694 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L).
Mouse Anti-MYC Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS1497694

Immunoprecipitation (IP) of anti-MYC antibody

Immunoprecipitating MYC-tag with transfected HEK293
Lane 1: Mouse control IgG (1ug)instead of MBS1497694 in transfected HEK293 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS1497694 (6ug)+ transfected HEK293 whole cell lysate (1mg)
Lane 3: Transfected HEK293 whole cell lysate (10ug)
Mouse Anti-MYC Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS1497694

Other Notes

Small volumes of anti-MYC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYC purchase

MBS1497694 is an antibody targeting MYC. Also known as Mouse Anti-MYC monoclonal Antibody. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MYC. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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