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Podocalyxin (PODXL) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1498612

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Podocalyxin (PODXL), Monoclonal Antibody
Full Product Name
Podocalyxin; GCTM-2 antigen; Gp200; PC; PODXL; PCLP; PCLP1
Product Gene Name
anti-PODXL antibody
Clonality
Monoclonal
Isotype
IgG2b
Host
Mouse
Species Reactivity
Human. Other species are not tested. Please decide the specificity by homology
Form/Format
Liquid
Purity/Purification
>95%, Protein G Purified
Specificity
Specific for PODXL denatured and native forms
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Molecular Weight
55,386 Da
OMIM
602632
3D Structure
ModBase 3D Structure for O00592
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant Human PODXL protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-23904 / sc-21705 / sc-21706 / sc-23903
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PODXL antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-PODXL antibody
Recommended dilution:
WB:1:500-1:5000
IHC:1:50-1:500
IF:1:50-1:200
IP:1:200-1:2000

Western Blot (WB) of anti-PODXL antibody

Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate
All lanes: PODXL antibody at 2.5 ug/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Mouse Anti-PODXL Monoclonal Antibody – Cat# MBS1498612

Western Blot (WB) of anti-PODXL antibody

Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: PODXL antibody at 2.5 ug/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Mouse Anti-PODXL Monoclonal Antibody, Western Blot (WB) – Cat# MBS1498612

Western Blot (WB) of anti-PODXL antibody

Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: PODXL antibody at 0.43 ug/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Mouse Anti-PODXL Monoclonal Antibody, Western Blot (WB) – Cat# MBS1498612

Western Blot (WB) of anti-PODXL antibody

Western Blot
Positive WB detected in: Rat brain tissue,Rat heart tissue,Rat kidney tissue
All lanes: PODXL antibody at 1.28 ug/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Mouse Anti-PODXL Monoclonal Antibody, Western Blot (WB) – Cat# MBS1498612

Immunohistochemistry (IHC) of anti-PODXL antibody

Immunohistochemistry of paraffin-embedded human kidney tissue at dilution of 1:200.
Mouse Anti-PODXL Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1498612

Immunohistochemistry (IHC) of anti-PODXL antibody

Immunohistochemistry of paraffin-embedded human melanoma at dilution of 1:200.
Mouse Anti-PODXL Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1498612

Immunofluorescence (IF) of anti-PODXL antibody

Immunofluorescent analysis of Hela cells at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
Mouse Anti-PODXL Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS1498612

Immunofluorescence (IF) of anti-PODXL antibody

Immunofluorescent analysis of PC-3 cells at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
Mouse Anti-PODXL Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS1498612

Immunofluorescence (IF) of anti-PODXL antibody

Immunofluorescent analysis of A549 cells at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
Mouse Anti-PODXL Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS1498612

Immunofluorescence (IF) of anti-PODXL antibody

Immunofluorescent analysis of Ntera2 cells at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
Mouse Anti-PODXL Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS1498612

Testing Data of anti-PODXL antibody

Overlay histogram showing Hela cells stained with MBS1498612 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IGg2b (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.
Mouse Anti-PODXL Monoclonal Antibody, Testing Data – Cat# MBS1498612

Immunoprecipitation (IP) of anti-PODXL antibody

Immunoprecipitating PODXL in HEK293 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1 ug)instead of MBS1498612 in HEK293 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS1498612 (8 ug)+ HEK293 whole cell lysate (500 ug)
Lane 3: HEK293 whole cell lysate (10 ug)
Mouse Anti-PODXL Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS1498612

Other Notes

Small volumes of anti-PODXL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PODXL purchase

MBS1498612 is an antibody targeting PODXL. Also known as Podocalyxin; GCTM-2 antigen; Gp200; PC; PODXL; PCLP; PCLP1. Reactive in Human. Other species are not tested. Please decide the specificity by homology. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PODXL. It may not necessarily be applicable to this product.

NCBI GI #
66277202
NCBI GeneID
5420
NCBI Accession #
NP_001018121.1
UniProt Primary Accession #
O00592
UniProt Secondary Accession #
Q52LZ7; Q53ER6; A6NHX8
UniProt Related Accession #
O00592
GenBank Nucleotide #
NM_001018111.2
NCBI Official Full Name
podocalyxin isoform 1
NCBI Official Synonym Full Names
podocalyxin like
NCBI Official Symbol
PODXL
NCBI Official Synonym Symbols
PC; PCLP; Gp200; PCLP-1
UniProt Protein Name
Podocalyxin
UniProt Synonym Protein Names
GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1
Protein Family
Podocalyxin
UniProt Gene Name
PODXL
UniProt Synonym Gene Names
PCLP; PCLP1; PC; PCLP-1
UniProt Entry Name
PODXL_HUMAN
NCBI Protein Information
podocalyxin

NCBI Summary for PODXL

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]
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UniProt Comments for PODXL

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 7q32-q33

Cellular Component: apical plasma membrane; cytoplasm; extracellular space; filopodium; integral to plasma membrane; intracellular membrane-bound organelle; lamellipodium; lipid raft; microvillus membrane; plasma membrane; ruffle

Molecular Function: protein binding

Biological Process: cell adhesion; cell migration; leukocyte migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; positive regulation of cell migration; positive regulation of cell-cell adhesion mediated by integrin; regulation of microvillus biogenesis
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Research Articles on PODXL

1. miR-125b was found to be important in arteriosclerosis obliterans by suppressing the expression of PODXL and may serve as a potential therapeutic target for the treatment of arteriosclerosis obliterans.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PODXL antibody

 Reference Product  PubMed Publications
 WT1 antibody  >4 publications with PODXL and WT1
 UBC antibody  >2 publications with PODXL and UBC
 SELL antibody  >1 publications with PODXL and SELL
 SPAG9 antibody  >1 publications with PODXL and SPAG9
 EZR antibody  >1 publications with PODXL and EZR

Diseases associated with anti-PODXL antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >12 publications with PODXL and Carcinoma
 Kidney Diseases Antibodies  >10 publications with PODXL and Kidney Diseases
 Liver Neoplasms Antibodies  >6 publications with PODXL and Liver Neoplasms
 Lung Neoplasms Antibodies  >5 publications with PODXL and Lung Neoplasms
 Liver Diseases Antibodies  >4 publications with PODXL and Liver Diseases
 Breast Neoplasms Antibodies  >4 publications with PODXL and Breast Neoplasms
 Nervous System Diseases Antibodies  >3 publications with PODXL and Nervous System Diseases
 Brain Diseases Antibodies  >3 publications with PODXL and Brain Diseases
 Cardiovascular Diseases Antibodies  >3 publications with PODXL and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >3 publications with PODXL and Disease Models, Animal

Organs/Tissues associated with anti-PODXL antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >20 publications with PODXL and Kidney
 Blood Antibodies  >18 publications with PODXL and Blood
 Vascular Antibodies  >17 publications with PODXL and Vascular
 Bone Antibodies  >12 publications with PODXL and Bone
 Embryonic Tissue Antibodies  >12 publications with PODXL and Embryonic Tissue
 Muscle Antibodies  >6 publications with PODXL and Muscle
 Lung Antibodies  >6 publications with PODXL and Lung
 Liver Antibodies  >6 publications with PODXL and Liver
 Heart Antibodies  >6 publications with PODXL and Heart
 Brain Antibodies  >4 publications with PODXL and Brain
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