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KLHL15 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150054

KLHL15 Antibody: KLHL15 is a member of a growing superfamily of kelch repeat-containing proteins. The kelch motif was discovered in the sequence of the Drosophila kelch ORF1 protein and forms a conserved beta-propeller tertiary structure. These kelch-like… More details

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Product Overview

Online Datasheet in PDF
Product Name
KLHL15, Polyclonal Antibody
Full Product Name
KLHL15 Antibody
Product Gene Name
Product Synonym Names
KLHL15; HEL-S-305; KIAA1677; Kelch-like protein 15; kelch-like 15 (Drosophila)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
KLHL15 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
KLHL15 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
KLHL15 peptide (MBS152935) is used for blocking the activity of KLHL15 antibody (MBS150054)
Molecular Weight
69,775 Da
3D Structure
ModBase 3D Structure for Q96M94
Conjugate
Unconjugated
Immunogen
KLHL15 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human KLHL15.
Buffer
KLHL15 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-KLHL15 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-KLHL15 antibody
KLHL15 antibody can be used for detection of KLHL15 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-KLHL15 antibody

Western blot analysis of KLHL15 in HeLa cell lysate with KLHL15 antibody at (A) 1 and (B) 2 μg/mL.
Rabbit Anti-KLHL15 Polyclonal Antibody – Cat# MBS150054

Immunocytochemistry (IHC) of anti-KLHL15 antibody

Immunocytochemistry of KLHL15 in HeLa cells with KLHL15 antibody at 5 μg/mL.
Rabbit Anti-KLHL15 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150054

Immunofluorescence (IF) of anti-KLHL15 antibody

Immunofluorescence of KLHL15 in HeLa cells with KLHL15 antibody at 20 μg/mL.
Rabbit Anti-KLHL15 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150054

Other Notes

Small volumes of anti-KLHL15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLHL15 purchase

MBS150054 is an antibody targeting KLHL15. Also known as KLHL15 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KLHL15. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
kelch-like protein 15
NCBI Official Synonym Full Names
kelch-like family member 15
NCBI Official Symbol
KLHL15
NCBI Official Synonym Symbols
HEL-S-305
UniProt Protein Name
Kelch-like protein 15
Protein Family
UniProt Gene Name
KLHL15
UniProt Synonym Gene Names
KIAA1677
UniProt Entry Name
KLH15_HUMAN
NCBI Protein Information
kelch-like protein 15; kelch-like 15; epididymis secretory protein Li 305

NCBI Summary for KLHL15

This gene encodes a member of the kelch-like family of proteins that share a common domain structure consisting of an N-terminal broad-complex, tramtrack, bric-a-brac/poxvirus and zinc finger domain and C-terminal kelch repeat motifs. The encoded protein may be involved in protein ubiquitination and cytoskeletal organization. [provided by RefSeq, Apr 2009]
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UniProt Comments for KLHL15

KLHL15: a substrate-specific adapter for the recruitment of substrates to Cul3-based E3 ubiquitin ligases. KLHL15-Cul3 specifically targets the PP2A regulatory subunit PPP2R5B to promote its turnover by ubiquitylation and proteasomal degradation. May contribute to the selective expression of the PP2A/B holoenzyme in the brain.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xp22.1-p21

Biological Process: protein ubiquitination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-KLHL15 antibody

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