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TFF3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150083

MBS150083 is an antibody targeting TFF3. Also known as TFF3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TFF3, Polyclonal Antibody
Full Product Name
TFF3 Antibody
Product Gene Name
Product Synonym Names
TFF3; ITF; P1B; TFI; ITF; Trefoil factor 3; Intestinal trefoil factor; hITF; trefoil factor 3 (intestinal)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid; TFF3 Antibody is supplied in PBS containing 0.02% sodium azide.
Purity/Purification
TFF3 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
TFF3 antibody is predicted to not cross-react with other TFF family members.
Preparation and Storage
TFF3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
TFF3 peptide (MBS152999) is used for blocking the activity of TFF3 antibody (MBS150083)
Molecular Weight
Predicted: 10 kDa

Observed: 11 kDa
OMIM
600633
3D Structure
ModBase 3D Structure for Q07654
Immunogen
TFF3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3.
The immunogen is located within the last 50 amino acids of TFF3
Homology
Predicted Species reactivity based on immunogen sequence: Bovine (81%), Pig: (81%)
Applications Tested/Suitable for anti-TFF3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC)

Western Blot (WB) of anti-TFF3 antibody

Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-TFF3 Polyclonal Antibody – Cat# MBS150083

Immunohistochemistry (IHC) of anti-TFF3 antibody

Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2.5 μg/mL.
Rabbit Anti-TFF3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150083

Immunofluorescence (IF) of anti-TFF3 antibody

Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 μg/mL.
Rabbit Anti-TFF3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150083

Immunocytochemistry (ICC) of anti-TFF3 antibody

Immunocytochemistry of TFF3 in HeLa cells with TFF3 antibody at 2 μg/ml.
Rabbit Anti-TFF3 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS150083

Immunofluorescence (IF) of anti-TFF3 antibody

Immunofluorescence of TFF3 in human small intestine tissue with TFF3 antibody at 20 μg/ml.

Red: TFF3 Antibody (6113)
Blue: DAPI staining
Rabbit Anti-TFF3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150083

Immunohistochemistry (IHC) of anti-TFF3 antibody

Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2 μg/ml.
Rabbit Anti-TFF3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150083

Other Notes

Small volumes of anti-TFF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TFF3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
trefoil factor 3 (intestinal), isoform CRA_b
NCBI Official Synonym Full Names
trefoil factor 3 (intestinal)
NCBI Official Symbol
TFF3
NCBI Official Synonym Symbols
ITF; P1B; TFI
UniProt Protein Name
Trefoil factor 3
UniProt Synonym Protein Names
Intestinal trefoil factor; hITF; Polypeptide P1.B; hP1.B
Protein Family
UniProt Gene Name
TFF3
UniProt Synonym Gene Names
ITF; TFI; hITF; hP1.B
UniProt Entry Name
TFF3_HUMAN
NCBI Protein Information
trefoil factor 3; polypeptide P1.B

NCBI Summary for TFF3

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
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UniProt Comments for TFF3

TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region; secretory granule

Biological Process: response to peptide hormone stimulus; digestion; defense response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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