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SPRED3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150128

Spred3 Antibody: Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Spred3, Polyclonal Antibody
Full Product Name
Spred3 Antibody
Product Gene Name
Product Synonym Names
Spred3; Eve-3; spred-3; EVE-3; Sprouty-related; EVH1 domain-containing protein 3; Spred-3; sprouty-related, EVH1 domain containing 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Spred3 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.
Preparation and Storage
Spred3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
Spred3 peptide (MBS152887) is used for blocking the activity of Spred3 antibody (MBS150128)
Molecular Weight
16,330 Da
OMIM
609293
3D Structure
ModBase 3D Structure for Q2MJR0
Conjugate
Unconjugated
Immunogen
Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3.
Buffer
Spred3 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-SPRED3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-SPRED3 antibody
Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-SPRED3 antibody

Western blot analysis of Spred3 in mouse brain tissue lysate with Spred3 antibody at (A) 2 and (B) 4 μg/mL.
Rabbit Anti-SPRED3 Polyclonal Antibody – Cat# MBS150128

Immunohistochemistry (IHC) of anti-SPRED3 antibody

Immunohistochemistry of Spred3 in human brain tissue with Spred3 antibody at 2.5 μg/mL.
Rabbit Anti-SPRED3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150128

Immunofluorescence (IF) of anti-SPRED3 antibody

Immunofluorescence of spred3 in human brain tissue with spred3 antibody at 20 μg/mL.
Rabbit Anti-SPRED3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150128

Other Notes

Small volumes of anti-SPRED3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPRED3 purchase

MBS150128 is an antibody targeting SPRED3. Also known as Spred3 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPRED3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sprouty-related, EVH1 domain-containing protein 3
NCBI Official Synonym Full Names
sprouty-related, EVH1 domain containing 3
NCBI Official Symbol
SPRED3
NCBI Official Synonym Symbols
Eve-3; spred-3
UniProt Protein Name
Sprouty-related, EVH1 domain-containing protein 3
UniProt Gene Name
SPRED3
UniProt Synonym Gene Names
EVE-3; Spred-3
UniProt Entry Name
SPRE3_HUMAN
NCBI Protein Information
sprouty-related, EVH1 domain-containing protein 3

NCBI Summary for SPRED3

This gene encodes a protein with a C-terminal Sprouty-like cysteine-rich domain (SRY) and an N-terminal Ena/Vasodilator-stimulated phosphoprotein (VASP) homology-1 (EVH-1) domain. The encoded protein is a member of a family of proteins that negatively regulates mitogen-activated protein (MAP) kinase signaling, particularly during organogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
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UniProt Comments for SPRED3

SPRED3: Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: plasma membrane

Biological Process: multicellular organismal development; inactivation of MAPK activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPRED3 antibody

Pathways associated with anti-SPRED3 antibody

Diseases associated with anti-SPRED3 antibody

 Disease Name  Pubmed Publications
 Glioma Antibodies  >1 publications with SPRED3 and Glioma
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