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PIWI-L3 (PIWIL3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150178

PIWI-L3 Antibody: PIWI-L3 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem… More details

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Product Overview

Online Datasheet in PDF
Product Name
PIWI-L3 (PIWIL3), Polyclonal Antibody
Full Product Name
PIWI-L3 Antibody
Product Gene Name
anti-PIWIL3 antibody
Product Synonym Names
PIWI-L3; HIWI3; Piwi-like protein 3; piwi-like 3 (Drosophila)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
PIWI-L3 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
PIWI-L3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
PIWI-L3 peptide (MBS151820) is used for blocking the activity of PIWI-L3 antibody (MBS150178)
Molecular Weight
101,089 Da
OMIM
610314
3D Structure
ModBase 3D Structure for Q7Z3Z3
Conjugate
Unconjugated
Immunogen
PIWI-L3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L3.
Buffer
PIWI-L3 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-PIWIL3 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-PIWIL3 antibody
PIWI-L3 antibody can be used for detection of PIWI-L3 by Western blot at 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-PIWIL3 antibody

Western blot analysis of PIWI-L3 in 3T3 cell lysate with PIWI-L3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-PIWIL3 Polyclonal Antibody – Cat# MBS150178

Immunofluorescence (IF) of anti-PIWIL3 antibody

Immunofluorescence of PIWI-L3 in 3T3 cells with PIWI-L3 antibody at 20 μg/mL.
Rabbit Anti-PIWIL3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150178

Other Notes

Small volumes of anti-PIWIL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIWIL3 purchase

MBS150178 is an antibody targeting PIWIL3. Also known as PIWI-L3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PIWIL3. It may not necessarily be applicable to this product.

NCBI GI #
153792298
NCBI GeneID
440822
NCBI Accession #
NP_001008496
UniProt Primary Accession #
Q7Z3Z3
UniProt Related Accession #
Q7Z3Z3
GenBank Nucleotide #
NM_001008496.3
NCBI Official Full Name
piwi-like protein 3 isoform 1
NCBI Official Synonym Full Names
piwi-like RNA-mediated gene silencing 3
NCBI Official Symbol
PIWIL3
NCBI Official Synonym Symbols
HIWI3
UniProt Protein Name
Piwi-like protein 3
Protein Family
Piwi-like protein
UniProt Gene Name
PIWIL3
UniProt Entry Name
PIWL3_HUMAN
NCBI Protein Information
piwi-like protein 3; piwi-like 3

NCBI Summary for PIWIL3

This gene encodes a member of the PIWI subfamily of Argonaute family proteins. This subfamily of proteins contains a PAZ domain, found in proteins involved in RNA-mediated gene silencing, and a C-terminal Piwi domain. The encoded protein is thought to function in maintenance of germline cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
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UniProt Comments for PIWIL3

PIWIL3: May play a role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Belongs to the argonaute family. Piwi subfamily.

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cytoplasm

Molecular Function: RNA binding

Biological Process: regulation of translation; meiotic cell cycle; multicellular organismal development; spermatogenesis; RNA-mediated gene silencing; cell differentiation
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Research Articles on PIWIL3

1. individuals with HIWI3 non-synonymous rs11703684 variant genotypes exhibited a significantly reduced oligozoospermia risk

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PIWIL3 antibody

 Products by Pathway  Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram

Diseases associated with anti-PIWIL3 antibody

 Disease Name  Pubmed Publications
 Colorectal Neoplasms Antibodies  >1 publications with PIWIL3 and Colorectal Neoplasms
 Colonic Neoplasms Antibodies  >1 publications with PIWIL3 and Colonic Neoplasms
 Infertility, Male Antibodies  >1 publications with PIWIL3 and Infertility, Male
 Oligospermia Antibodies  >1 publications with PIWIL3 and Oligospermia

Organs/Tissues associated with anti-PIWIL3 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with PIWIL3 and Testis
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