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NENF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150227

NENF Antibody: NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated… More details

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Product Overview

Online Datasheet in PDF
Product Name
NENF, Polyclonal Antibody
Full Product Name
NENF Antibody
Product Gene Name
Product Synonym Names
NENF; CIR2; SPUF; SCIRP10; CIR2; Neudesin; Cell immortalization-related protein 2; SPUF protein; neuron derived neurotrophic factor
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
NENF Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
NENF antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
NENF peptide (MBS153206) is used for blocking the activity of NENF antibody (MBS150227)
Molecular Weight
18,856 Da
OMIM
611874
3D Structure
ModBase 3D Structure for Q9UMX5
Conjugate
Unconjugated
Immunogen
NENF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF.
Buffer
NENF Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-NENF antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-NENF antibody
NENF antibody can be used for detection of NENF by Western blot at 1 - 2 mug/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-NENF antibody

Western blot analysis of NENF in human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 μg/mL.
Rabbit Anti-NENF Polyclonal Antibody – Cat# MBS150227

Immunohistochemistry (IHC) of anti-NENF antibody

Immunohistochemistry of NENF in rat kidney tissue with NENF antibody at 10 μg/mL.
Rabbit Anti-NENF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150227

Immunofluorescence (IF) of anti-NENF antibody

Immunofluorescence of NENF in rat kidney tissue with NENF antibody at 20 μg/mL.
Rabbit Anti-NENF Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150227

Other Notes

Small volumes of anti-NENF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NENF purchase

MBS150227 is an antibody targeting NENF. Also known as NENF Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NENF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neudesin
NCBI Official Synonym Full Names
neudesin neurotrophic factor
NCBI Official Symbol
NENF
NCBI Official Synonym Symbols
CIR2; SPUF; SCIRP10
UniProt Protein Name
Neudesin
UniProt Synonym Protein Names
Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Secreted protein of unknown function; SPUF protein
Protein Family
UniProt Gene Name
NENF
UniProt Synonym Gene Names
CIR2; SPUF; SPUF protein
UniProt Entry Name
NENF_HUMAN
NCBI Protein Information
neudesin; SCIRP10-related protein; cell growth-inhibiting protein 47; neuron-derived neurotrophic factor; secreted protein of unknown function; Spinal cord injury related protein 10; cell immortalization-related protein 2

NCBI Summary for NENF

This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2009]

UniProt Comments for NENF

NENF: Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme. Belongs to the cytochrome b5 family. MAPR subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleoplasm; extracellular space; membrane; endomembrane system; nucleus

Molecular Function: growth factor activity; metal ion binding; heme binding

Biological Process: positive regulation of MAPKKK cascade; negative regulation of appetite
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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