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NLRC3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150315

NOD3 Antibody: NOD3 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD3 also known as NLR family CARD containing 3 (NLRC3), is predominantly expressed in the immune… More details

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Product Overview

Online Datasheet in PDF
Product Name
NOD3 (NLRC3), Polyclonal Antibody
Full Product Name
NOD3 Antibody
Product Gene Name
Product Synonym Names
NOD3; NOD3; CLR16.2; NOD3; Protein NLRC3; CARD15-like protein; NLR family, CARD domain containing 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
NOD3 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
NOD3 peptide (MBS152024) is used for blocking the activity of NOD3 antibody (MBS150315)
Molecular Weight
111,634 Da
3D Structure
ModBase 3D Structure for Q7RTR2
Conjugate
Unconjugated
Immunogen
NOD3 antibody was raised against an 18 amino acid synthetic peptide near the center of human NOD3.
Buffer
NOD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-NLRC3 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-NLRC3 antibody
NOD3 antibody can be used for detection of NOD3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-NLRC3 antibody

Western blot analysis of NOD3 in 3T3 cell lysate with NOD3 antibody at (A) 1 and (B) 2 μg/mL.
Rabbit Anti-NLRC3 Polyclonal Antibody – Cat# MBS150315

Immunofluorescence (IF) of anti-NLRC3 antibody

Immunofluorescence of NOD3 in Jurkat cells with NOD3 antibody at 20 μg/mL.
Rabbit Anti-NLRC3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150315

Other Notes

Small volumes of anti-NLRC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NLRC3 purchase

MBS150315 is an antibody targeting NLRC3. Also known as NOD3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NLRC3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
NOD3 protein, isoform CRA_d
NCBI Official Synonym Full Names
NLR family, CARD domain containing 3
NCBI Official Symbol
NLRC3
NCBI Official Synonym Symbols
NOD3; CLR16.2
UniProt Protein Name
Protein NLRC3
UniProt Synonym Protein Names
CARD15-like protein; Caterpiller protein 16.2; CLR16.2; Nucleotide-binding oligomerization domain protein 3
Protein Family
UniProt Gene Name
NLRC3
UniProt Synonym Gene Names
NOD3; CLR16.2
UniProt Entry Name
NLRC3_HUMAN
NCBI Protein Information
protein NLRC3; CARD15-like protein; NOD-like receptor C3; caterpiller protein 16.2; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3

NCBI Summary for NLRC3

This gene encodes a NOD-like receptor family member. The encoded protein is a cytosolic regulator of innate immunity. This protein directly interacts with stimulator of interferon genes (STING), to prevent its proper trafficking, resulting in disruption of STING-dependent activation of the innate immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
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UniProt Comments for NLRC3

NLRC3: May modulate T-cell activation. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm

Molecular Function: ATP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; T cell activation; regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; response to lipopolysaccharide; negative regulation of I-kappaB kinase/NF-kappaB cascade
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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