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SPRED2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150328

Spred2 Antibody: Spred2 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1, Spred2 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Spred2, Polyclonal Antibody
Full Product Name
Spred2 Antibody
Product Gene Name
Product Synonym Names
Spred2; Spred-2; Sprouty-related; EVH1 domain-containing protein 2; Spred-2; sprouty-related, EVH1 domain containing 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Spred2 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.
Preparation and Storage
Spred2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
Spred2 peptide (MBS152931) is used for blocking the activity of Spred2 antibody (MBS150328)
Molecular Weight
47,033 Da
OMIM
609292
3D Structure
ModBase 3D Structure for Q7Z698
Conjugate
Unconjugated
Immunogen
Spred2 antibody was raised against a 13 amino acid synthetic peptide near the center of the human Spred2.
Buffer
Spred2 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-SPRED2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-SPRED2 antibody
Spred2 antibody can be used for detection of Spred2 by Western blot at 1 - 2 mug/mL.

Western Blot (WB) of anti-SPRED2 antibody

Western blot analysis of Spred2 in human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 μg/mL.
Rabbit Anti-SPRED2 Polyclonal Antibody – Cat# MBS150328

Other Notes

Small volumes of anti-SPRED2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPRED2 purchase

MBS150328 is an antibody targeting SPRED2. Also known as Spred2 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPRED2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sprouty-related, EVH1 domain-containing protein 2 isoform a
NCBI Official Synonym Full Names
sprouty-related, EVH1 domain containing 2
NCBI Official Symbol
SPRED2
NCBI Official Synonym Symbols
Spred-2
UniProt Protein Name
Sprouty-related, EVH1 domain-containing protein 2
UniProt Gene Name
SPRED2
UniProt Synonym Gene Names
Spred-2
UniProt Entry Name
SPRE2_HUMAN
NCBI Protein Information
sprouty-related, EVH1 domain-containing protein 2; sprouty protein with EVH-1 domain 2, related sequence

NCBI Summary for SPRED2

SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008]

UniProt Comments for SPRED2

SPRED2: Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p14

Cellular Component: nucleoplasm; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase inhibitor activity; protein binding; stem cell factor receptor binding; protein kinase binding

Biological Process: multicellular organismal development; positive regulation of DNA damage response, signal transduction by p53 class mediator; inactivation of MAPK activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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