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DCAF12 Antibody – Chicken Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150399

TCC52 Antibody: Cancer-testis (CT) antigens are a type of antigen whose expression is highly restricted, primarily in normal testis, but aberrantly in different tumor types. Their immunogenicity in cancer patients makes them prime targets for immunotherapy of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TCC52 (DCAF12), Polyclonal Antibody
Full Product Name
TCC52 Antibody
Product Gene Name
Product Synonym Names
TCC52; CT102; TCC52; WDR40A; KIAA1892; DDB1- and CUL4-associated factor 12; Centrosome-related protein TCC52; DDB1 and CUL4 associated factor 12
Clonality
Polyclonal
Isotype
IgY
Host
Chicken
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
TCC52 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
TCC52 peptide (MBS152539) is used for blocking the activity of TCC52 antibody (MBS150399)
Molecular Weight
50,517 Da
3D Structure
ModBase 3D Structure for Q5T6F0
Conjugate
Unconjugated
Immunogen
TCC52 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TCC52.
Buffer
TCC52 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-DCAF12 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-DCAF12 antibody
TCC52 antibody can be used for detection of TCC52 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL.

Western Blot (WB) of anti-DCAF12 antibody

Western blot analysis of TCC52 in 293 cell lysate with TCC52 antibody at 1 μg/mL.
Chicken Anti-DCAF12 Polyclonal Antibody – Cat# MBS150399

Immunocytochemistry (IHC) of anti-DCAF12 antibody

Immunocytochemistry of TCC52 in 293 cells with TCC52 antibody at 2.5 μg/mL.
Chicken Anti-DCAF12 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150399

Other Notes

Small volumes of anti-DCAF12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DCAF12 purchase

MBS150399 is an antibody targeting DCAF12. Also known as TCC52 Antibody. Reactive in Human, Mouse, Rat. Host: Chicken.

Additional Details

NCBI/Uniprot data below describe general gene information for DCAF12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DDB1- and CUL4-associated factor 12
NCBI Official Synonym Full Names
DDB1 and CUL4 associated factor 12
NCBI Official Symbol
DCAF12
NCBI Official Synonym Symbols
CT102; TCC52; WDR40A; KIAA1892
UniProt Protein Name
DDB1- and CUL4-associated factor 12
UniProt Synonym Protein Names
Centrosome-related protein TCC52; Testis cancer centrosome-related protein; WD repeat-containing protein 40A
UniProt Gene Name
DCAF12
UniProt Synonym Gene Names
KIAA1892; TCC52; WDR40A
UniProt Entry Name
DCA12_HUMAN
NCBI Protein Information
DDB1- and CUL4-associated factor 12; WD repeat domain 40A; cancer/testis antigen 102; WD repeat-containing protein 40A; centrosome-related protein TCC52; testis cancer centrosome-related protein

NCBI Summary for DCAF12

This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. [provided by RefSeq, Jul 2009]

UniProt Comments for DCAF12

WDR40A: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Belongs to the WD repeat DCAF12 family.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: centrosome; cytoplasm

Biological Process: protein ubiquitination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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