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RIPK3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150424

RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RIP3 (Ripk3), Polyclonal Antibody
Full Product Name
RIP3 Antibody
Product Gene Name
anti-Ripk3 antibody
Product Synonym Names
RIP3; Rip3; AW107945; 2610528K09Rik; Rip3; RIP-like protein kinase 3; RIP-3; receptor-interacting serine-threonine kinase 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
RIP3 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
RIP3 peptide (MBS152640) is used for blocking the activity of RIP3 antibody (MBS150424)
Molecular Weight
Predicted: 53 kD for mouse RIP3 and 57kD for human RIP3
Observed: 53 kD for mouse RIP3 and 57kD for human RIP3
3D Structure
ModBase 3D Structure for Q9QZL0
Conjugate
Unconjugated
Immunogen
Anti-RIP3 antibody (MBS150424) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of murine RIP3.The immunogen is located within the last 50 amino acids of RIP3.
Buffer
RIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Isoforms
Mouse RIP3 has one isoform (486aa, 53kD). Human RIP3 has 3 isoforms, including isoform1 (518aa, 57kD), isoform 2 (252aa, 28kD) and isoform 3 (231aa, 25kD). Rat RIP3 also hasone isoform (478aa, 52kD). MBS150424 can detect can detect isoforms of mouse and rat as well as human isoform 1.
Validation
KO Validation
(Figure 7,9,13) shows RIP3 expression detected by anti-RIP3 antibodies (MBS150424) was disrupted in multiple tissues of RIP3 KO mice (figure 7, 13) and in RIP3 KO MEF cells (figure 9).
KD Validation
(Figure 10): Anti-RIP3 antibody (MBS150424) specificity was further verified by RIP3 specific knockdown. RIP3 signal in L929 cells transfected with RIP3 shRNA was disrupted in comparison with that in cells transfected with control shRNA.
Regulated Expression Validation
(Figure 7,11,13): RIP3 expression detected by anit-RIP3 antibodies (MBS150424) was up-regulated by treatment of cerulein (figure 7), LPS (figure 11) or acetaminophen (figure 13) in different tissues or cells.
Overexpression Validation
(Figure 12): RIP3 overexpression detected by anit-RIP3 antibodies (MBS150424) was observed in three cell lines stably transfected with RIP3.
Positive Control
1) Cat. No. MBS151754 – A431 Cell Lysate
2) Cat. No. MBS151593 - 3T3/NIH Cell Lysate
3) Cat. No. MBS151658- C2C12 Cell Lysate
4) Cat. No. MBS151757- 3T3/Balb Cell Lysate
Applications Tested/Suitable for anti-Ripk3 antibody
ELISA, IF, IHC-P, IP, WB
Application Notes for anti-Ripk3 antibody
WB: 0.1-0.5 ug/mL
IHC-P: 5 ug/mL
IF: 20 ug/mL
IP: 20 ug/mL

Antibody validated: Western Blot in mouse and human samples; Immunohistochemistryin mouse and rat samples; Immunofluorescence in mouse and rat samples;Immunoprecipitation in human samples; Immunogold EM in mouse samples. All otherapplications and species not yet tested

Western Blot (WB) of anti-Ripk3 antibody

Loading: 15 ?g of lysates per lane. Antibodies: RIP3 (MBS150424), (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution
Rabbit Anti-RIPK3 Polyclonal Antibody – Cat# MBS150424

Western Blot (WB) of anti-Ripk3 antibody

Loading: 15 ug of lysates per lane. Antibodies: RIP3 MBS150424, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: MBS150424, 0.1 ug/mL in the presence of peptide blocking Lane 2: MBS150424, 0.1 ug/mL Lane 3: MBS150424, 0.2 ug/mL Lane 4: MBS150424,0.5 ug/mL
Rabbit Anti-RIPK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS150424

Western Blot (WB) of anti-Ripk3 antibody

Loading: 15 ug of lysates per lane. Antibodies: RIP3 MBS150424,(0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Rabbit Anti-RIPK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS150424

Immunohistochemistry (IHC) of anti-Ripk3 antibody

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-RIP3 antibody (MBS150424) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 uC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.
Rabbit Anti-RIPK3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150424

Immunohistochemistry (IHC) of anti-Ripk3 antibody

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-RIP3 antibody (MBS150424) at 2.5ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 uC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.
Rabbit Anti-RIPK3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150424

Immunofluorescence (IF) of anti-Ripk3 antibody

Immunofluorescent analysis of 4% paraformaldehyde-fixed Rat Kidney tissue labeling RIP3 with MBS150424 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Rabbit Anti-RIPK3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150424

Western Blot (WB) of anti-Ripk3 antibody

Western blot analysis of RIP3 with anti-RIP3 antibodies shows disrupted RIP3 expression in pancreas, liver spleen thymus and lung of RIP3 KO mice. Cerulein treatment upregulated RIP3 expression in the pancreas of WT mice.
Rabbit Anti-RIPK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS150424

Immunohistochemistry (IHC) of anti-Ripk3 antibody

RIP3 expression detected by anti-RIP3 antibodies (MBS150424) was decreased in Fadd KO mice as compared to WT mice.
Rabbit Anti-RIPK3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150424

Western Blot (WB) of anti-Ripk3 antibody

Western blot analysis of RIP3 with anti-RIP3 antibodies shows disrupted RIP3 expression in RIP3 KO MEFs, but not in WT MEF cells.
Rabbit Anti-RIPK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS150424

Testing Data (TD) of anti-Ripk3 antibody

Immunoblot analysis of RIP3 with anti-RIP3 antibodies (MBS150424) shows RIP3 expression was disrupted in RIP3 knockdown L929 cells.
Rabbit Anti-RIPK3 Polyclonal Antibody, Testing Data (TD) – Cat# MBS150424

Testing Data (TD) of anti-Ripk3 antibody

Confocal microscopy shows colocalization of RIPK1 (green) and RIPK3 (red) in PM and RIPK3 was detected by anti-RIPK3 antibodies (MBS150424). PF or BCM suppressed LPS-induced upregulation of RIP3.
Rabbit Anti-RIPK3 Polyclonal Antibody, Testing Data (TD) – Cat# MBS150424

Testing Data (TD) of anti-Ripk3 antibody

The cancer cell lines were stably expressing DYKDDDDK-tagged RIP3 and RIP expression was detected by anti-RIP3 antibodies (MBS150424) in RIP3-overexpressed cells.
Rabbit Anti-RIPK3 Polyclonal Antibody, Testing Data (TD) – Cat# MBS150424

Other Notes

Small volumes of anti-Ripk3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ripk3 purchase

MBS150424 is an antibody targeting Ripk3. Also known as RIP3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Ripk3. It may not necessarily be applicable to this product.

NCBI GI #
6063101
NCBI GeneID
56532
NCBI Accession #
AAF03133
UniProt Primary Accession #
Q9QZL0
UniProt Secondary Accession #
Q3U3Z9; Q8K2Y2; G3X8V8
UniProt Related Accession #
Q9QZL0
NCBI Official Full Name
receptor interacting protein 3
NCBI Official Synonym Full Names
receptor-interacting serine-threonine kinase 3
NCBI Official Symbol
Ripk3
NCBI Official Synonym Symbols
Rip3; AW107945; 2610528K09Rik
UniProt Protein Name
Receptor-interacting serine/threonine-protein kinase 3
UniProt Synonym Protein Names
RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3; mRIP3
Protein Family
Receptor-interacting serine/threonine-protein kinase
UniProt Gene Name
Ripk3
UniProt Synonym Gene Names
Rip3; RIP-3; mRIP3
UniProt Entry Name
RIPK3_MOUSE
NCBI Protein Information
receptor-interacting serine/threonine-protein kinase 3; RIP-3; mRIP3; RIP-like protein kinase 3; receptor interacting protein 3; receptor-interacting protein 3

UniProt Comments for Ripk3

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; RIPK family

Cellular Component: mitochondrion; membrane; cytoplasm; plasma membrane; intracellular; cytosol

Molecular Function: transferase activity; NF-kappaB-inducing kinase activity; identical protein binding; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: spleen development; regulation of activated T cell proliferation; I-kappaB kinase/NF-kappaB cascade; viral reproduction; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; T cell differentiation in the thymus; programmed cell death; positive regulation of transferase activity; lymph node development; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; activation of protein kinase activity; T cell homeostasis; positive regulation of ligase activity; phosphorylation; protein homooligomerization; positive regulation of oxidoreductase activity
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Research Articles on Ripk3

1. our results reveal a specific role for the RIP1-RIP3-DRP1 pathway in RNA virus-induced activation of the NLRP3 inflammasome

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Ripk3 antibody

 Reference Product  PubMed Publications
 Ripk1 antibody  >87 publications with Ripk3 and Ripk1
 Casp8 antibody  >73 publications with Ripk3 and Casp8
 Fadd antibody  >41 publications with Ripk3 and Fadd
 Mlkl antibody  >40 publications with Ripk3 and Mlkl
 Tnfrsf1a antibody  >9 publications with Ripk3 and Tnfrsf1a
 Tlr4 antibody  >6 publications with Ripk3 and Tlr4
 Zbp1 antibody  >5 publications with Ripk3 and Zbp1
 Tlr3 antibody  >4 publications with Ripk3 and Tlr3
 Ticam1 antibody  >3 publications with Ripk3 and Ticam1

Pathways associated with anti-Ripk3 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 IKK Complex Recruitment Mediated By RIP1 Pathway antibodies  IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 MyD88-independent Cascade Pathway antibodies  MyD88-independent Cascade Pathway Diagram
 RIP-mediated NFkB Activation Via ZBP1 Pathway antibodies  RIP-mediated NFkB Activation Via ZBP1 Pathway Diagram
 TNF Signaling Pathway antibodies  TNF Signaling Pathway Diagram

Diseases associated with anti-Ripk3 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >174 publications with Ripk3 and Necrosis
 Inflammation Antibodies  >68 publications with Ripk3 and Inflammation
 Cardiovascular Diseases Antibodies  >12 publications with Ripk3 and Cardiovascular Diseases
 Liver Diseases Antibodies  >12 publications with Ripk3 and Liver Diseases
 Drug-Induced Liver Injury Antibodies  >6 publications with Ripk3 and Drug-Induced Liver Injury
 Lung Diseases Antibodies  >4 publications with Ripk3 and Lung Diseases
 Drug-Related Side Effects and Adverse Reactions Antibodies  >4 publications with Ripk3 and Drug-Related Side Effects and Adverse Reactions
 Liver Neoplasms Antibodies  >3 publications with Ripk3 and Liver Neoplasms
 Acute Kidney Injury Antibodies  >3 publications with Ripk3 and Acute Kidney Injury
 Carcinoma, Hepatocellular Antibodies  >2 publications with Ripk3 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-Ripk3 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >19 publications with Ripk3 and Liver
 Bone Antibodies  >13 publications with Ripk3 and Bone
 Intestine Antibodies  >11 publications with Ripk3 and Intestine
 Bone Marrow Antibodies  >11 publications with Ripk3 and Bone Marrow
 Brain Antibodies  >9 publications with Ripk3 and Brain
 Lung Antibodies  >8 publications with Ripk3 and Lung
 Eye Antibodies  >7 publications with Ripk3 and Eye
 Embryonic Tissue Antibodies  >7 publications with Ripk3 and Embryonic Tissue
 Heart Antibodies  >6 publications with Ripk3 and Heart
 Skin Antibodies  >5 publications with Ripk3 and Skin
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