Rabbit Anti-DFFA Polyclonal Antibody – Cat# MBS150439

Western blot analysis of DFF45 in HeLa cell lysate with DFF45 antibody at (A) 1 and (B) 2 μg/mL.

DFF45 (DFFA) Antibody – Rabbit Polyclonal

Name: DFFA Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS150439
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150439

DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death… More details

$530 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DFF45 (DFFA), Polyclonal Antibody

Full Product Name

DFF45 Antibody

Product Gene Name

anti-DFFA antibody

Product Synonym Names

DFF45; DFF1; ICAD; DFF-45; DFF1; DFF45; H13; DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor, 45kDa, alpha polypeptide

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

DFF45 Antibody is Antibody is DEAE purified.

Preparation and Storage

DFF45 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Antibody/Peptide Pairs

DFF45 peptide (MBS152513) is used for blocking the activity of DFF45 antibody (MBS150439)

Molecular Weight

45; 35 kDa

OMIM

601882

3D Structure

ModBase 3D Structure for O00273

Conjugate

Unconjugated

Immunogen

DFF45 antibody was raised against a 20 amino acid peptide near the amino terminus of human DFF45.

Buffer

DFF45 Antibody is supplied in PBS containing 0.02% sodium azide.

Applications Tested/Suitable for anti-DFFA antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-DFFA antibody

DFF45 antibody can be used for detection of DFF45, DFF35, and one the cleaved fragment by Western blot at 1:1000 to 1:2000 dilution. 45 and 35 kDa bands can be detected in non-apoptotic cells.

Other Notes

Small volumes of anti-DFFA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DFFA purchase

MBS150439 is an antibody targeting DFFA. Also known as DFF45 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DFFA. It may not necessarily be applicable to this product.

NCBI GI #

4758148

NCBI GeneID

1676

NCBI Accession #

NP_004392

UniProt Primary Accession #

O00273

UniProt Secondary Accession #

Q5T6G5; Q5T6G6; Q96I97; Q9Y6C6

UniProt Related Accession #

O00273

GenBank Nucleotide #

NM_004401.2

NCBI Official Full Name

DNA fragmentation factor subunit alpha isoform 1

NCBI Official Synonym Full Names

DNA fragmentation factor, 45kDa, alpha polypeptide

NCBI Official Symbol

DFFA

NCBI Official Synonym Symbols

DFF1; ICAD; DFF-45

UniProt Protein Name

DNA fragmentation factor subunit alpha

UniProt Synonym Protein Names

DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD

Protein Family

DNA fragmentation factor

UniProt Gene Name

DFFA

UniProt Synonym Gene Names

DFF1; DFF45; DFF-45; ICAD

UniProt Entry Name

DFFA_HUMAN

NCBI Protein Information

DNA fragmentation factor subunit alpha; DFF45; inhibitor of CAD; DNA fragmentation factor 45 kDa subunit

NCBI Summary for DFFA

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for DFFA

DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; lipid particle; nucleus; cytosol

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis

Research Articles on DFFA

1. Data suggest that DFF45 gene silencing, when applied in combination with doxorubicin, may offer a therapeutic strategy for the treatment of breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DFFA antibody

Reference Product	PubMed Publications
CIDEB antibody	>8 publications with DFFA and CIDEB
DFFB antibody	>6 publications with DFFA and DFFB
CASP3 antibody	>4 publications with DFFA and CASP3
MYC antibody	>3 publications with DFFA and MYC

Pathways associated with anti-DFFA antibody

Products by Pathway	Pathway Diagram
Activation Of DNA Fragmentation Factor Pathway antibodies	Activation Of DNA Fragmentation Factor Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
Apoptosis Induced DNA Fragmentation Pathway antibodies	Apoptosis Induced DNA Fragmentation Pathway Diagram
Apoptotic Execution Phase Pathway antibodies	Apoptotic Execution Phase Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
FAS Pathway And Stress Induction Of HSP Regulation antibodies	FAS Pathway And Stress Induction Of HSP Regulation Diagram

Diseases associated with anti-DFFA antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>8 publications with DFFA and Necrosis
Carcinoma Antibodies	>5 publications with DFFA and Carcinoma
Fatty Liver Antibodies	>5 publications with DFFA and Fatty Liver
Liver Diseases Antibodies	>4 publications with DFFA and Liver Diseases
Cardiovascular Diseases Antibodies	>4 publications with DFFA and Cardiovascular Diseases
Disease Models, Animal Antibodies	>3 publications with DFFA and Disease Models, Animal
Inflammation Antibodies	>3 publications with DFFA and Inflammation
Uterine Cervical Neoplasms Antibodies	>2 publications with DFFA and Uterine Cervical Neoplasms
Nervous System Diseases Antibodies	>2 publications with DFFA and Nervous System Diseases
Neoplasm Invasiveness Antibodies	>1 publications with DFFA and Neoplasm Invasiveness

Organs/Tissues associated with anti-DFFA antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>11 publications with DFFA and Liver
Connective Tissue Antibodies	>9 publications with DFFA and Connective Tissue
Blood Antibodies	>6 publications with DFFA and Blood
Muscle Antibodies	>4 publications with DFFA and Muscle
Heart Antibodies	>3 publications with DFFA and Heart
Kidney Antibodies	>3 publications with DFFA and Kidney
Bone Antibodies	>3 publications with DFFA and Bone
Bone Marrow Antibodies	>2 publications with DFFA and Bone Marrow
Vascular Antibodies	>2 publications with DFFA and Vascular
Eye Antibodies	>2 publications with DFFA and Eye