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MSI2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150550

MSI2 Antibody: Musashi2 (MSI2) is an RNA-binding protein that is highly expressed in precursor cells in the ventricular and subventricular zones of the developing mammalian CNS. Like the related MSI1, MSI2 has been suggested to be involved stem cell… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MSI2, Polyclonal Antibody
Full Product Name
MSI2 Antibody
Product Gene Name
Product Synonym Names
MSI2; MSI2H; RNA-binding protein Musashi homolog 2; Musashi-2; musashi homolog 2 (Drosophila)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid; MSI2 Antibody is supplied in PBS containing 0.02% sodium azide.
Purity/Purification
MSI2 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
MSI2 antibody is predicted to not cross-react with MSI1. Multiple isoforms of MSI2 are known to exist.
Preparation and Storage
MSI2 antibody can be stored at 4 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
MSI2 peptide (MBS152042) is used for blocking the activity of MSI2 antibody (MBS150550)
OMIM
607897
3D Structure
ModBase 3D Structure for Q96DH6
Conjugate
Unconjugated
Immunogen
MSI2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MSI2.
The immunogen is located within amino acids 30 - 80 of MSI2.
Positive Control
MBS151675 - EL4 Cell Lysate
MBS154295 - EL4 Cell Slide
Applications Tested/Suitable for anti-MSI2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P)
Application Notes for anti-MSI2 antibody
MSI2 antibody can be used for detection of MSI2 by Western blot at 1 ug/mL. Antibodycan also be used for immunohistochemistry starting at 5 ug/mL andimmunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 5 ug/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mousesamples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples.

All other applications and species not yet tested.

Western Blot (WB) of anti-MSI2 antibody

MSI2 Antibody
Rabbit Anti-MSI2 Polyclonal Antibody – Cat# MBS150550

Immunocytochemistry (IHC) of anti-MSI2 antibody

Immunocytochemistry of MSI2 in EL4 cells with MSI2 antibody at 5 μg/mL.
Rabbit Anti-MSI2 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150550

Immunofluorescence (IF) of anti-MSI2 antibody

Immunofluorescence of MSI2 in EL4 cells with MSI2 antibody at 20 μg/mL.
Rabbit Anti-MSI2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150550

Immunohistochemistry (IHC) of anti-MSI2 antibody

Immunohistochemistry of MSI2 in mouse brain tissue with MSI2 Antibody at 5 μg/mL.
Rabbit Anti-MSI2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150550

Immunofluorescence (IF) of anti-MSI2 antibody

Immunofluorescence of MSI2 in mouse brain tissue with MSI2 Antibody at 20 μg/mL.

Green: MSI2 antibody (6309)
Red: Phylloidin staining
Blue: DAPI staining
Rabbit Anti-MSI2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150550

Other Notes

Small volumes of anti-MSI2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSI2 purchase

MBS150550 is an antibody targeting MSI2. Also known as MSI2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MSI2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RNA-binding protein Musashi homolog 2 isoform a
NCBI Official Synonym Full Names
musashi RNA-binding protein 2
NCBI Official Symbol
MSI2
NCBI Official Synonym Symbols
MSI2H
UniProt Protein Name
RNA-binding protein Musashi homolog 2
Protein Family
UniProt Gene Name
MSI2
UniProt Synonym Gene Names
Musashi-2
UniProt Entry Name
MSI2H_HUMAN
NCBI Protein Information
RNA-binding protein Musashi homolog 2; musashi-2; musashi homolog 2

NCBI Summary for MSI2

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for MSI2

Musashi-2: RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system. Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23). Belongs to the Musashi family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: polysome; cytoplasm

Molecular Function: nucleotide binding; poly(U) binding

Biological Process: stem cell development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MSI2 antibody

Pathways associated with anti-MSI2 antibody

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