Phospho-Nuclear Factor Kappa B (PNFKB) ELISA Kit for Fish

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PNFKB concentrations in Fish serum, plasma and other body fluids. Using Purified Fish PNFKB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PNFKB and PNFKB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PNFKB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of PNFKB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS015059 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospho-Nuclear Factor Kappa B (PNFKB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PNFKB. The ELISA analytical biochemical technique of the MBS015059 kit is based on PNFKB antibody-PNFKB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PNFKB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PNFKB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Intended use of this ELISA Kit is quantitative determination of fish phospho-nuclear factor kappa B (PNFKB), protein involved in DNA transcription control, cytokine production and regulation of immune response.

Test system is designed as sandwich-type ELISA and concentration of PNFKB is measured using pair of antibodies specific to target protein.

Kit is applicable for testing variable types of samples, including plasma, serum and other bodily fluids, and also tissue homogenates.

Product contains microwell plate pre-coated with purified capture antibodies against PNFKB and all reagents required for its quantification, including sample diluent and wash solution.

Assay kits provide colorimetric detection of PNFKB using detector antibodies against PNFKB labeled with HRP as signaling molecule, and TMB as its substrate solution.

Minimal detectable concentration of ELISA kit is 10pg/mL and its linear range is 62.5-2,000pg/mL; calibrators for standard curve include six levels.

Intra- and inter-assay CV values, reported as kit’s precision characteristics, are not greater than 15%.

Assay doesn’t show significant cross-reactivity with analogous proteins.

ELISA kit and its reagents and components require refrigeration and must be stored at 2-8 degree C until expiration date (approximately six months from its manufacturing).