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LRRFIP2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150675

LRRFIP2 Antibody: The leucine-rich repeat FLI-I-interacting protein 2 (LRRFIP2), like the related protein LRRFIP1, was identified in a yeast two-hybrid system through binding to the LRR domain of human FLI. It can activate the Wnt signaling pathway in… More details

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Product Overview

Online Datasheet in PDF
Product Name
LRRFIP2, Polyclonal Antibody
Full Product Name
LRRFIP2 Antibody
Product Gene Name
Product Synonym Names
LRRFIP2; HUFI-2; Leucine-rich repeat flightless-interacting protein 2; LRR FLII-interacting protein 2; leucine rich repeat (in FLII) interacting protein 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
LRRFIP2 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
Three isoforms of LRRFIP2 are known to exist; this antibody will recognize all three. LRRFIP2 antibody is predicted to not cross-react with other LRRFIP family members.
Preparation and Storage
LRRFIP2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
LRRFIP2 peptide (MBS151868) is used for blocking the activity of LRRFIP2 antibody (MBS150675)
OMIM
614043
3D Structure
ModBase 3D Structure for Q9Y608
Conjugate
Unconjugated
Immunogen
LRRFIP2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human LRRFIP2. The immunogen is located within amino acids 620-760 of LRRFIP2
Buffer
LRRFIP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Applications Tested/Suitable for anti-LRRFIP2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Application Notes for anti-LRRFIP2 antibody
LRRFIP2 antibody can be used for detection of LRRFIP2 by Western blot at 0.5 and 1 ug/mL.
Antibody can also be used for immunohistochemistry starting at 5 ug/mL.
For immunofluorescence start at 20 ug/mL.
Positive Control: MBS151749 Rat Colon Tissue Lysate

Optimal dilutions for each application to be determined by the researcher.

Western Blot (WB) of anti-LRRFIP2 antibody

Western blot analysis of LRRFIP2 in rat colon tissue lysate with LRRFIP2 antibody at (A) 0.5 and (B) 1 μg/mL.
Rabbit Anti-LRRFIP2 Polyclonal Antibody – Cat# MBS150675

Immunohistochemistry (IHC) of anti-LRRFIP2 antibody

Immunohistochemistry of LRRFIP2 in human colon tissue with LRRFIP2 antibody at 5 μg/mL.
Rabbit Anti-LRRFIP2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150675

Immunofluorescence (IF) of anti-LRRFIP2 antibody

Immunofluorescence of LRRFIP2 in human colon tissue with LRRFIP2 antibody at 20 μg/mL.
Rabbit Anti-LRRFIP2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150675

Other Notes

Small volumes of anti-LRRFIP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LRRFIP2 purchase

MBS150675 is an antibody targeting LRRFIP2. Also known as LRRFIP2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LRRFIP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
leucine-rich repeat flightless-interacting protein 2 isoform 1
NCBI Official Synonym Full Names
leucine rich repeat (in FLII) interacting protein 2
NCBI Official Symbol
LRRFIP2
NCBI Official Synonym Symbols
HUFI-2
UniProt Protein Name
Leucine-rich repeat flightless-interacting protein 2
UniProt Gene Name
LRRFIP2
UniProt Synonym Gene Names
LRR FLII-interacting protein 2
UniProt Entry Name
LRRF2_HUMAN
NCBI Protein Information
leucine-rich repeat flightless-interacting protein 2; LRR FLII-interacting protein 2

UniProt Comments for LRRFIP2

LRRFIP2: May function as activator of the canonical Wnt signaling pathway, in association with DVL3, upstream of CTNNB1/beta- catenin. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding. Belongs to the LRRFIP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 3p22.2

Molecular Function: protein binding; LRR domain binding

Biological Process: Wnt receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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