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TRMT12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150690

TYW2 Antibody: TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TYW2 (TRMT12), Polyclonal Antibody
Full Product Name
TYW2 Antibody
Product Gene Name
Product Synonym Names
TYW2; TYW2; TRM12; TYW2; tRNA wybutosine-synthesizing protein 2 homolog; Alpha-amino-alpha-carboxypropyl transferase TYW2; tRNA-yW-synthesizing protein 2; tRNA methyltransferase 12 homolog
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
TYW2 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms
Preparation and Storage
TYW2 antibody can be stored at 4 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
TYW2 peptide (MBS152812) is used for blocking the activity of TYW2 antibody (MBS150690)
Molecular Weight
50,236 Da
OMIM
611244
3D Structure
ModBase 3D Structure for Q53H54
Conjugate
Unconjugated
Immunogen
TYW2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2.
Buffer
TYW2 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-TRMT12 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-TRMT12 antibody
TYW2 antibody can be used for detection of TYW2 by Western blot at 0.5 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.

Western Blot (WB) of anti-TRMT12 antibody

Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-TRMT12 Polyclonal Antibody – Cat# MBS150690

Immunocytochemistry (IHC) of anti-TRMT12 antibody

Immunocytochemistry of TYW2 in K562 cells with TYW2 antibody at 10 μg/mL.
Rabbit Anti-TRMT12 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150690

Other Notes

Small volumes of anti-TRMT12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRMT12 purchase

MBS150690 is an antibody targeting TRMT12. Also known as TYW2 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRMT12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tRNA wybutosine-synthesizing protein 2 homolog
NCBI Official Synonym Full Names
tRNA methyltransferase 12 homolog (S. cerevisiae)
NCBI Official Symbol
TRMT12
NCBI Official Synonym Symbols
TYW2; TRM12
UniProt Protein Name
tRNA wybutosine-synthesizing protein 2 homolog
UniProt Synonym Protein Names
tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase
UniProt Gene Name
TRMT12
UniProt Synonym Gene Names
TRM12; TYW2; tRNA-yW-synthesizing protein 2
UniProt Entry Name
TYW2_HUMAN
NCBI Protein Information
tRNA wybutosine-synthesizing protein 2 homolog; tRNA-yW synthesizing protein 2; tRNA-yW-synthesizing protein 2; tRNA methyltranferase 12 homolog; homolog of yeast tRNA methyltransferase; alpha-amino-alpha-carboxypropyl transferase TYW2; tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase

NCBI Summary for TRMT12

Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008]
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UniProt Comments for TRMT12

TRMT12: Probable S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Belongs to the TRM5 / TYW2 family.

Protein type: Methyltransferase; EC 2.5.1.114

Chromosomal Location of Human Ortholog: 8q24.13

Molecular Function: transferase activity

Biological Process: tRNA processing
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-TRMT12 antibody

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