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M6PRBP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150702

TIP47 Antibody: Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TIP47 (M6PRBP1), Polyclonal Antibody
Full Product Name
TIP47 Antibody
Product Gene Name
Product Synonym Names
TIP47; PP17; TIP47; M6PRBP1; Perilipin-3; 47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; mannose-6-phosphate receptor binding protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
TIP47 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
TIP47 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
TIP47 peptide (MBS153215) is used for blocking the activity of TIP47 antibody (MBS150702)
Molecular Weight
Predicted: 48 kDa

Observed: 53 kDa
OMIM
602702
3D Structure
ModBase 3D Structure for O60664
Conjugate
Unconjugated
Immunogen
TIP47 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TIP47.
Buffer
TIP47 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-M6PRBP1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-M6PRBP1 antibody
TIP47 antibody can be used for the detection of TIP47 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL.

Western Blot (WB) of anti-M6PRBP1 antibody

Western blot analysis of TIP47 in EL4 cell lysate with TIP47 antibody at (A) 0.5 and (B) 1 μg/mL.
Rabbit Anti-M6PRBP1 Polyclonal Antibody – Cat# MBS150702

Immunohistochemistry (IHC) of anti-M6PRBP1 antibody

Immunohistochemistry of TIP47 in rat liver tissue with TIP47 antibody at 2 μg/mL.
Rabbit Anti-M6PRBP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150702

Other Notes

Small volumes of anti-M6PRBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for M6PRBP1 purchase

MBS150702 is an antibody targeting M6PRBP1. Also known as TIP47 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for M6PRBP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Perilipin-3
NCBI Official Synonym Full Names
perilipin 3
NCBI Official Symbol
PLIN3
NCBI Official Synonym Symbols
PP17; TIP47; M6PRBP1
UniProt Protein Name
Perilipin-3
UniProt Synonym Protein Names
47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; Mannose-6-phosphate receptor-binding protein 1; Placental protein 17; PP17
Protein Family
UniProt Gene Name
PLIN3
UniProt Synonym Gene Names
M6PRBP1; TIP47; 47 kDa MPR-binding protein; PP17
UniProt Entry Name
PLIN3_HUMAN
NCBI Protein Information
perilipin-3; placental protein 17; 47 kDa MPR-binding protein; cargo selection protein TIP47; tail-interacting protein, 47 kD; mannose-6-phosphate receptor-binding protein 1

NCBI Summary for M6PRBP1

Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009]
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UniProt Comments for M6PRBP1

perilipin 3: a membrane-associated protein and a coat protein for lipid droplets (LDs). It has been described as a mannose-6-phosphate receptor (MPR) binding protein required for endosome-to-Golgi transport of MPRs, but this function has been disputed. Interacts with M6PR and IGF2R via their cytoplasmic domains. Affects hepatic lipid and glucose metabolism. Belongs to the perilipin family. 3 isoforms of the human protein are produced by alternative splicing. Isoform 2 may exist as a homodimer known as PP17C.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; membrane; cytoplasm; endosome membrane; lipid particle; endosome

Molecular Function: protein binding

Biological Process: vesicle-mediated transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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