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ADAP (FYB) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150722

ADAP Antibody: The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ADAP (FYB), Polyclonal Antibody
Full Product Name
ADAP Antibody
Product Gene Name
Product Synonym Names
ADAP; ADAP; PRO0823; SLAP-130; SLAP130; FYN-binding protein; Adhesion and degranulation promoting adaptor protein; ADAP; FYN binding protein (FYB-120/130)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
ADAP Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
ADAP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
ADAP peptide (MBS152728) is used for blocking the activity of ADAP antibody (MBS150722)
Molecular Weight
91,651 Da
OMIM
602731
3D Structure
ModBase 3D Structure for O15117
Conjugate
Unconjugated
Immunogen
ADAP antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human ADAP.
Buffer
ADAP Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-FYB antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-FYB antibody
ADAP antibody can be used for detection of ADAP by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-FYB antibody

Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 μg/mL.
Rabbit Anti-FYB Polyclonal Antibody – Cat# MBS150722

Immunocytochemistry (IHC) of anti-FYB antibody

Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 μg/mL.
Rabbit Anti-FYB Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150722

Other Notes

Small volumes of anti-FYB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FYB purchase

MBS150722 is an antibody targeting FYB. Also known as ADAP Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FYB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
FYN-binding protein isoform 1
NCBI Official Synonym Full Names
FYN binding protein
NCBI Official Symbol
FYB
NCBI Official Synonym Symbols
ADAP; PRO0823; SLAP130; SLAP-130
UniProt Protein Name
FYN-binding protein
UniProt Synonym Protein Names
Adhesion and degranulation promoting adaptor protein; ADAP; FYB-120/130; p120/p130; FYN-T-binding protein; SLAP-130; SLP-76-associated phosphoprotein
Protein Family
UniProt Gene Name
FYB
UniProt Synonym Gene Names
SLAP130; ADAP; p120/p130
UniProt Entry Name
FYB_HUMAN
NCBI Protein Information
FYN-binding protein; p120/p130; FYB-120/130; FYN-T-binding protein; SLP-76-associated phosphoprotein; adhesion and degranulation-promoting adaptor protein

NCBI Summary for FYB

The protein encoded by this gene is an adapter for the FYN protein and LCP2 signaling cascades in T-cells. The encoded protein is involved in platelet activation and controls the expression of interleukin-2. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
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UniProt Comments for FYB

SLAP-130: an adapter protein for fyn and the SH2-domain-containing leucocyte protein-76 (SLP76) in T cells. T-cell receptor ligation leads to increased tyrosine phosphorylation of SLAP-130. Two alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: cytosol; nucleus; actin cytoskeleton

Molecular Function: protein binding; protein complex binding; receptor binding

Biological Process: immune response; NLS-bearing substrate import into nucleus; signal transduction; T cell receptor signaling pathway; protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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