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OCLN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150725

OCLN Antibody: Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
OCLN, Polyclonal Antibody
Full Product Name
OCLN Antibody
Product Gene Name
anti-OCLN antibody
Product Synonym Names
OCLN; BLCPMG; Occludin; occludin
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
OCLN Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
OCLN peptide (MBS152755) is used for blocking the activity of OCLN antibody (MBS150725)
Molecular Weight
8,175 Da
OMIM
phenotype 602876
3D Structure
ModBase 3D Structure for Q16625
Conjugate
Unconjugated
Immunogen
OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN.
Buffer
OCLN Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-OCLN antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-OCLN antibody
OCLN antibody can be used for detection of OCLN by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-OCLN antibody

Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 μg/mL.
Rabbit Anti-OCLN Polyclonal Antibody – Cat# MBS150725

Immunohistochemistry (IHC) of anti-OCLN antibody

Immunohistochemistry of OCLN in human liver tissue with OCLN antibody at 2.5 μg/mL.
Rabbit Anti-OCLN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150725

Immunofluorescence (IF) of anti-OCLN antibody

Immunofluorescence of OCLN in Human Liver tissue with OCLN antibody at 20 μg/mL.
Rabbit Anti-OCLN Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150725

Immunofluorescence (IF) of anti-OCLN antibody

Immunofluorescence of OCLN in human liver tissue with OCLN antibody at 20 μg/ml.

Red: OCLN Antibody (5191)
Blue: DAPI staining
Rabbit Anti-OCLN Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150725

Immunofluorescence (IF) of anti-OCLN antibody

Immunofluorescence of OCLN in mouse brain tissue with OCLN antibody at 20 μg/mL.

Red: OCLN Antibody (5191)
Blue: DAPI staining
Rabbit Anti-OCLN Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150725

Immunohistochemistry (IHC) of anti-OCLN antibody

Immunohistochemistry of OCLN in mouse brain tissue with OCLN Antibody at 5 μg/mL.
Rabbit Anti-OCLN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150725

Other Notes

Small volumes of anti-OCLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OCLN purchase

MBS150725 is an antibody targeting OCLN. Also known as OCLN Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.

NCBI GI #
20987418
NCBI GeneID
4950
NCBI Accession #
AAH29886
UniProt Primary Accession #
Q16625
UniProt Secondary Accession #
Q5U1V4; Q8N6K1; B5BU70; D2DU64; D2DU65; D2IGC0; D2IGC1; E2CYV9
NCBI Official Full Name
OCLN protein
UniProt Protein Name
Occludin
Protein Family
Occludin
UniProt Gene Name
OCLN
UniProt Entry Name
OCLN_HUMAN

UniProt Comments for OCLN

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: tight junction; apicolateral plasma membrane; endocytic vesicle; apical plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle; intercellular junction; cell junction; cytosol

Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity

Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; protein complex assembly; cell structure disassembly during apoptosis

Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-OCLN antibody

 Reference Product  PubMed Publications
 TJP1 antibody  >526 publications with OCLN and TJP1
 CLDN1 antibody  >274 publications with OCLN and CLDN1
 CLDN5 antibody  >131 publications with OCLN and CLDN5
 CLDN4 antibody  >64 publications with OCLN and CLDN4
 CLDN3 antibody  >48 publications with OCLN and CLDN3
 TJP2 antibody  >48 publications with OCLN and TJP2
 CLDN7 antibody  >17 publications with OCLN and CLDN7
 TJP3 antibody  >14 publications with OCLN and TJP3
 CGN antibody  >7 publications with OCLN and CGN
 YES1 antibody  >1 publications with OCLN and YES1

Pathways associated with anti-OCLN antibody

 Products by Pathway  Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with anti-OCLN antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >140 publications with OCLN and Inflammation
 Disease Models, Animal Antibodies  >118 publications with OCLN and Disease Models, Animal
 Necrosis Antibodies  >82 publications with OCLN and Necrosis
 Cardiovascular Diseases Antibodies  >81 publications with OCLN and Cardiovascular Diseases
 Edema Antibodies  >60 publications with OCLN and Edema
 Liver Diseases Antibodies  >43 publications with OCLN and Liver Diseases
 Retinal Diseases Antibodies  >33 publications with OCLN and Retinal Diseases
 Colitis Antibodies  >29 publications with OCLN and Colitis
 Kidney Diseases Antibodies  >15 publications with OCLN and Kidney Diseases
 Hemorrhage Antibodies  >14 publications with OCLN and Hemorrhage

Organs/Tissues associated with anti-OCLN antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >267 publications with OCLN and Brain
 Intestine Antibodies  >230 publications with OCLN and Intestine
 Liver Antibodies  >121 publications with OCLN and Liver
 Eye Antibodies  >102 publications with OCLN and Eye
 Kidney Antibodies  >71 publications with OCLN and Kidney
 Testis Antibodies  >56 publications with OCLN and Testis
 Embryonic Tissue Antibodies  >52 publications with OCLN and Embryonic Tissue
 Lung Antibodies  >47 publications with OCLN and Lung
 Stomach Antibodies  >19 publications with OCLN and Stomach
 Pancreas Antibodies  >15 publications with OCLN and Pancreas
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