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AFP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150728

AFP Antibody: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma. However, hereditary persistance of AFP may also be found… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
AFP, Polyclonal Antibody
Full Product Name
AFP Antibody
Product Gene Name
Product Synonym Names
AFP; FETA; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; alpha-fetoprotein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
AFP Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
AFP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
AFP peptide (MBS151909) is used for blocking the activity of AFP antibody (MBS150728)
OMIM
104150
3D Structure
ModBase 3D Structure for P02771
Conjugate
Unconjugated
Immunogen
AFP antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human AFP. The immunogen is located within amino acids 340-390 of AFP.
Buffer
AFP Antibody is supplied in PBS containing 0.02% sodium azide.
Homology
Predicted species reactivity based on immunogen sequence: Pig: (91%)
Applications Tested/Suitable for anti-AFP antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF)
Application Notes for anti-AFP antibody
AFP antibody can be used for detection of AFP by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.
Optimal dilutions for each application to be determined by the researcher.

Western Blot (WB) of anti-AFP antibody

Western blot analysis of AFP in fetal human liver tissue lysate with AFP antibody at (A) 1 μg/mL and (B) 2 μg/mL.
Rabbit Anti-AFP Polyclonal Antibody – Cat# MBS150728

Immunohistochemistry (IHC) of anti-AFP antibody

Immunohistochemistry of AFP in mouse liver tissue with AFP antibody at 2.5 μg/ml.
Rabbit Anti-AFP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150728

Immunofluorescence (IF) of anti-AFP antibody

Immunofluorescence of AFP in rat liver tissue with AFP antibody at 20 μg/mL.
Rabbit Anti-AFP Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150728

Immunohistochemistry (IHC) of anti-AFP antibody

Immunohistochemistry of AFP in rat liver tissue with AFP antibody at 10 μg/mL.
Rabbit Anti-AFP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150728

Immunofluorescence (IF) of anti-AFP antibody

Immunofluorescence of AFP in mouse liver tissue with AFP antibody at 20 μg/mL.

Red: AFP Antibody (5869)
Blue: DAPI staining
Rabbit Anti-AFP Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150728

Other Notes

Small volumes of anti-AFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AFP purchase

MBS150728 is an antibody targeting AFP. Also known as AFP Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AFP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Official Synonym Symbols
AFPD; FETA; HPAFP
UniProt Protein Name
Alpha-fetoprotein
UniProt Synonym Protein Names
Alpha-1-fetoprotein; Alpha-fetoglobulin
Protein Family
UniProt Gene Name
AFP
UniProt Synonym Gene Names
HPAFP
UniProt Entry Name
FETA_HUMAN
NCBI Protein Information
alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein

NCBI Summary for AFP

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
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UniProt Comments for AFP

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cytoplasm

Molecular Function: metal ion binding

Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle

Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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