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PAPPA2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150746

PLAC3 Antibody: PLAC3 (placenta-specific protein 3), also known as PAPP-A2, is a metalloproteinase that cleaves the insulin-like growth factor-binding protein (IGFBP)-5 in a IGF-independent fashion. It has been suggested that the cleavage of IGFBP-5 and to a… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PLAC3 (PAPPA2), Polyclonal Antibody
Full Product Name
PLAC3 Antibody
Product Gene Name
Product Synonym Names
PLAC3; PAPPE; PLAC3; PAPP-E; PAPP-A2; Pappalysin-2; Pregnancy-associated plasma protein A2; pappalysin 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
PLAC3 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
PLAC3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
PLAC3 peptide (MBS151906) is used for blocking the activity of PLAC3 antibody (MBS150746)
Molecular Weight
92,136 Da
3D Structure
ModBase 3D Structure for Q9BXP8
Conjugate
Unconjugated
Immunogen
PLAC3 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human PLAC3.
Buffer
PLAC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-PAPPA2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-PAPPA2 antibody
PLAC3 antibody can be used for detection of PLAC3 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-PAPPA2 antibody

Western blot analysis of PLAC3 in HeLa cell lysate with PLAC3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-PAPPA2 Polyclonal Antibody – Cat# MBS150746

Immunocytochemistry (IHC) of anti-PAPPA2 antibody

Immunocytochemistry of PLAC3 in HeLa cells with PLAC3 antibody at 10 μg/mL.
Rabbit Anti-PAPPA2 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150746

Immunofluorescence (IF) of anti-PAPPA2 antibody

Immunofluorescence of PLAC3 in HeLa cells with PLAC3 antibody at 20 μg/mL.
Rabbit Anti-PAPPA2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150746

Other Notes

Small volumes of anti-PAPPA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAPPA2 purchase

MBS150746 is an antibody targeting PAPPA2. Also known as PLAC3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAPPA2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pappalysin-2 isoform 1
NCBI Official Synonym Full Names
pappalysin 2
NCBI Official Symbol
PAPPA2
NCBI Official Synonym Symbols
PAPPE; PLAC3; PAPP-E; PAPP-A2
UniProt Protein Name
Pappalysin-2
UniProt Synonym Protein Names
Pregnancy-associated plasma protein A2; PAPP-A2; Pregnancy-associated plasma protein E1; PAPP-E
Protein Family
UniProt Gene Name
PAPPA2
UniProt Synonym Gene Names
PLAC3; PAPP-A2; PAPP-E
UniProt Entry Name
PAPP2_HUMAN
NCBI Protein Information
pappalysin-2; placenta-specific 3; pregnancy-associated plasma protein E1; pregnancy-associated plasma preproprotein-A2

NCBI Summary for PAPPA2

This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
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UniProt Comments for PAPPA2

PAPPA2: Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3. Belongs to the peptidase M43B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: membrane; extracellular region; intracellular

Molecular Function: metallopeptidase activity; zinc ion binding

Biological Process: cellular protein metabolic process; regulation of cell growth; cell differentiation; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PAPPA2 antibody

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