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POFUT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150751

POFUT1 Antibody: POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
POFUT1, Polyclonal Antibody
Full Product Name
POFUT1 Antibody
Product Gene Name
anti-POFUT1 antibody
Product Synonym Names
POFUT1; DDD2; FUT12; O-FUT; OFUCT1; O-Fuc-T; O-FucT-1; KIAA0180; GDP-fucose protein O-fucosyltransferase 1; Peptide-O-fucosyltransferase 1; protein O-fucosyltransferase 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
POFUT1 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
Preparation and Storage
POFUT1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
POFUT1 peptide (MBS152637) is used for blocking the activity of POFUT1 antibody (MBS150751)
Molecular Weight
22,338 Da
OMIM
gene 615327
3D Structure
ModBase 3D Structure for Q9H488
Conjugate
Unconjugated
Immunogen
POFUT1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1.
Buffer
POFUT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-POFUT1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-POFUT1 antibody
POFUT1 antibody can be used for the detection of POFUT1 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.

Western Blot (WB) of anti-POFUT1 antibody

Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 μg/mL.
Rabbit Anti-POFUT1 Polyclonal Antibody – Cat# MBS150751

Immunohistochemistry (IHC) of anti-POFUT1 antibody

Immunohistochemistry of POFUT1 in human heart tissue with POFUT antibody at 2.5 μg/mL.
Rabbit Anti-POFUT1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150751

Other Notes

Small volumes of anti-POFUT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POFUT1 purchase

MBS150751 is an antibody targeting POFUT1. Also known as POFUT1 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POFUT1. It may not necessarily be applicable to this product.

NCBI GI #
27436889
NCBI GeneID
23509
NCBI Accession #
NP_056167
UniProt Primary Accession #
Q9H488
UniProt Secondary Accession #
Q14685; Q5W185; Q9BW76; A8K4R8; E1P5M4
UniProt Related Accession #
Q9H488
GenBank Nucleotide #
NM_015352.1
NCBI Official Full Name
GDP-fucose protein O-fucosyltransferase 1 isoform 1
NCBI Official Synonym Full Names
protein O-fucosyltransferase 1
NCBI Official Symbol
POFUT1
NCBI Official Synonym Symbols
DDD2; FUT12; O-FUT; OFUCT1; O-Fuc-T; O-FucT-1
UniProt Protein Name
GDP-fucose protein O-fucosyltransferase 1
UniProt Synonym Protein Names
Peptide-O-fucosyltransferase 1; O-FucT-1
UniProt Gene Name
POFUT1
UniProt Synonym Gene Names
FUT12; KIAA0180; O-FucT-1
UniProt Entry Name
OFUT1_HUMAN
NCBI Protein Information
GDP-fucose protein O-fucosyltransferase 1; o-fucosyltransferase protein; peptide-O-fucosyltransferase 1

NCBI Summary for POFUT1

This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for POFUT1

POFUT1: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling. Belongs to the glycosyltransferase 68 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.1.221; Transferase

Chromosomal Location of Human Ortholog: 20q11

Cellular Component: membrane; endoplasmic reticulum

Molecular Function: peptide-O-fucosyltransferase activity; fucosyltransferase activity

Biological Process: fucose metabolic process; nervous system development; protein amino acid O-linked glycosylation; embryonic development; somitogenesis; Notch signaling pathway; O-glycan processing; regulation of transcription, DNA-dependent; heart development; angiogenesis

Disease: Dowling-degos Disease 2
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Research Articles on POFUT1

1. POFUT1 expression can contribute to cancer progression and that POFUT1 may serve as a diagnostic marker and a therapeutic target for OSCCs.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POFUT1 antibody

 Reference Product  PubMed Publications
 NOTCH1 antibody  >11 publications with POFUT1 and NOTCH1
 DLL1 antibody  >3 publications with POFUT1 and DLL1
 NOTCH2 antibody  >3 publications with POFUT1 and NOTCH2
 PLAGL2 antibody  >2 publications with POFUT1 and PLAGL2

Pathways associated with anti-POFUT1 antibody

 Products by Pathway  Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Other Types Of O-glycan Biosynthesis Pathway antibodies  Other Types Of O-glycan Biosynthesis Pathway Diagram
 Other Types Of O-glycan Biosynthesis Pathway antibodies  Other Types Of O-glycan Biosynthesis Pathway Diagram
 Pre-NOTCH Expression And Processing Pathway antibodies  Pre-NOTCH Expression And Processing Pathway Diagram
 Pre-NOTCH Processing In The Endoplasmic Reticulum Pathway antibodies  Pre-NOTCH Processing In The Endoplasmic Reticulum Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By NOTCH Pathway antibodies  Signaling By NOTCH Pathway Diagram

Diseases associated with anti-POFUT1 antibody

 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >3 publications with POFUT1 and Congenital Abnormalities
 Hyperplasia Antibodies  >3 publications with POFUT1 and Hyperplasia
 Embryo Loss Antibodies  >2 publications with POFUT1 and Embryo Loss
 Metaplasia Antibodies  >2 publications with POFUT1 and Metaplasia
 Cardiovascular Diseases Antibodies  >2 publications with POFUT1 and Cardiovascular Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with POFUT1 and Cell Transformation, Neoplastic
 Heart Defects, Congenital Antibodies  >1 publications with POFUT1 and Heart Defects, Congenital
 Adenocarcinoma Antibodies  >1 publications with POFUT1 and Adenocarcinoma
 Fetal Death Antibodies  >1 publications with POFUT1 and Fetal Death
 Inflammation Antibodies  >1 publications with POFUT1 and Inflammation

Organs/Tissues associated with anti-POFUT1 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >5 publications with POFUT1 and Skin
 Muscle Antibodies  >5 publications with POFUT1 and Muscle
 Embryonic Tissue Antibodies  >4 publications with POFUT1 and Embryonic Tissue
 Lung Antibodies  >4 publications with POFUT1 and Lung
 Blood Antibodies  >4 publications with POFUT1 and Blood
 Heart Antibodies  >3 publications with POFUT1 and Heart
 Ovary Antibodies  >2 publications with POFUT1 and Ovary
 Vascular Antibodies  >2 publications with POFUT1 and Vascular
 Bone Antibodies  >2 publications with POFUT1 and Bone
 Liver Antibodies  >2 publications with POFUT1 and Liver
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