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N4BP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150781

N4BP1 Antibody: Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
N4BP1, Polyclonal Antibody
Full Product Name
N4BP1 Antibody
Product Gene Name
Product Synonym Names
N4BP1; KIAA0615; NEDD4-binding protein 1; N4BP1; NEDD4 binding protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
N4BP1 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
Preparation and Storage
N4BP1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
N4BP1 peptide (MBS152853) is used for blocking the activity of N4BP1 antibody (MBS150781)
Molecular Weight
100,379 Da
3D Structure
ModBase 3D Structure for O75113
Conjugate
Unconjugated
Immunogen
N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1.
Buffer
N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-N4BP1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-N4BP1 antibody
N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-N4BP1 antibody

Western blot analysis of N4BP1 in HeLa cell lysate with N4BP1 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-N4BP1 Polyclonal Antibody – Cat# MBS150781

Immunocytochemistry (IHC) of anti-N4BP1 antibody

Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 μg/mL.
Rabbit Anti-N4BP1 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150781

Immunofluorescence (IF) of anti-N4BP1 antibody

Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 μg/mL.
Rabbit Anti-N4BP1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150781

Other Notes

Small volumes of anti-N4BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for N4BP1 purchase

MBS150781 is an antibody targeting N4BP1. Also known as N4BP1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for N4BP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NEDD4-binding protein 1
NCBI Official Synonym Full Names
NEDD4 binding protein 1
NCBI Official Symbol
N4BP1
UniProt Protein Name
NEDD4-binding protein 1
Protein Family
UniProt Gene Name
N4BP1
UniProt Synonym Gene Names
KIAA0615; N4BP1
UniProt Entry Name
N4BP1_HUMAN
NCBI Protein Information
NEDD4-binding protein 1

UniProt Comments for N4BP1

N4BP1: Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. Belongs to the N4BP1 family.

Protein type: Nucleolus; Unknown function

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: PML body; nucleolus

Molecular Function: protein binding

Biological Process: negative regulation of protein ubiquitination; negative regulation of proteasomal ubiquitin-dependent protein catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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