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KLHL25 Antibody – Chicken Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150793

ENC-2 Antibody: The ectoderm-neural cortex-2 (ENC-2) protein a member of the BTB/PDZ Kelch protein family displaying significant homology to ENC-1, an actin-binding protein involved in neural fate specification during embryogenesis and adipocyte maturation.… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ENC-2 (KLHL25), Polyclonal Antibody
Full Product Name
ENC-2 Antibody
Product Gene Name
anti-KLHL25 antibody
Product Synonym Names
ENC-2; ENC2; ENC-2; ENC2; Kelch-like protein 25; Ectoderm-neural cortex protein 2; kelch-like 25 (Drosophila)
Clonality
Polyclonal
Isotype
IgY
Host
Chicken
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
ENC-2 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
ENC-2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
ENC-2 peptide (MBS153240) is used for blocking the activity of ENC-2 antibody (MBS150793)
Molecular Weight
65,923 Da
3D Structure
ModBase 3D Structure for Q9H0H3
Conjugate
Unconjugated
Immunogen
ENC-2 antibody was raised against a 14 amino acid synthetic peptide near the center of human ENC-2.
Buffer
ENC-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-KLHL25 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-KLHL25 antibody
ENC-2 antibody can be used for detection of ENC-2 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-KLHL25 antibody

Western blot analysis of ENC-2 in Jurkat cell lysate with ENC-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Chicken Anti-KLHL25 Polyclonal Antibody – Cat# MBS150793

Immunocytochemistry (IHC) of anti-KLHL25 antibody

Immunocytochemistry of ENC-2 in Jurkat cells with ENC-2 antibody at 5 μg/mL.
Chicken Anti-KLHL25 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150793

Immunofluorescence (IF) of anti-KLHL25 antibody

Immunofluorescence of ENC-2 in Jurkat cells with ENC-2 antibody at 20 μg/mL.
Chicken Anti-KLHL25 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150793

Other Notes

Small volumes of anti-KLHL25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLHL25 purchase

MBS150793 is an antibody targeting KLHL25. Also known as ENC-2 Antibody. Reactive in Human. Host: Chicken.

Additional Details

NCBI/Uniprot data below describe general gene information for KLHL25. It may not necessarily be applicable to this product.

NCBI GI #
55418570
NCBI GeneID
64410
NCBI Accession #
AAV51405
UniProt Primary Accession #
Q9H0H3
UniProt Secondary Accession #
B2RDH2; B3KRT7
UniProt Related Accession #
Q9H0H3
NCBI Official Full Name
ENC2
NCBI Official Synonym Full Names
kelch-like family member 25
NCBI Official Symbol
KLHL25
NCBI Official Synonym Symbols
ENC2; ENC-2
UniProt Protein Name
Kelch-like protein 25
UniProt Synonym Protein Names
Ectoderm-neural cortex protein 2; ENC-2
Protein Family
Kelch-like protein
UniProt Gene Name
KLHL25
UniProt Synonym Gene Names
ENC2; ENC-2
UniProt Entry Name
KLH25_HUMAN
NCBI Protein Information
kelch-like protein 25; kelch-like 25; ectodermal-neural cortex 2; BTB/POZ KELCH domain protein; ectoderm-neural cortex protein 2

UniProt Comments for KLHL25

KLHL25: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E. Component of the BCR(KLHL25) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL25 and RBX1. Interacts with EIF4EBP1 (when hypophosphorylated)

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 15q25.3

Cellular Component: cytoplasm

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; regulation of translational initiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLHL25 antibody

 Reference Product  PubMed Publications
 EIF4EBP1 antibody  >1 publications with KLHL25 and EIF4EBP1
 CUL3 antibody  >1 publications with KLHL25 and CUL3
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