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VPS53 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150860

VPS53 Antibody: The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde… More details

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Product Overview

Online Datasheet in PDF
Product Name
VPS53, Polyclonal Antibody
Full Product Name
VPS53 Antibody
Product Gene Name
Product Synonym Names
VPS53; HCCS1; hVps53L; pp13624; Vacuolar protein sorting-associated protein 53 homolog; vacuolar protein sorting 53 homolog (S. cerevisiae)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
VPS53 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
VPS53 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
VPS53 peptide (MBS152307) is used for blocking the activity of VPS53 antibody (MBS150860)
Molecular Weight
94,405 Da
OMIM
gene 615851
3D Structure
ModBase 3D Structure for Q5VIR6
Conjugate
Unconjugated
Immunogen
VPS53 antibody was raised against a 18 amino acid synthetic peptide from near the center of human VPS53.
Buffer
VPS53 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-VPS53 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-VPS53 antibody
VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 - 1 mug/mL.

Western Blot (WB) of anti-VPS53 antibody

Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 μg/mL.
Rabbit Anti-VPS53 Polyclonal Antibody – Cat# MBS150860

Other Notes

Small volumes of anti-VPS53 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VPS53 purchase

MBS150860 is an antibody targeting VPS53. Also known as VPS53 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VPS53. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
vacuolar protein sorting 53 (yeast), isoform CRA_a
NCBI Official Synonym Full Names
vacuolar protein sorting 53 homolog (S. cerevisiae)
NCBI Official Symbol
VPS53
NCBI Official Synonym Symbols
HCCS1; PCH2E; hVps53L; pp13624
UniProt Protein Name
Vacuolar protein sorting-associated protein 53 homolog
UniProt Gene Name
VPS53
UniProt Entry Name
VPS53_HUMAN
NCBI Protein Information
vacuolar protein sorting-associated protein 53 homolog

NCBI Summary for VPS53

This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008]

UniProt Comments for VPS53

VPS53: May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Belongs to the VPS53 family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; endosome membrane

Molecular Function: protein binding

Biological Process: protein transport; retrograde transport, endosome to Golgi

Disease: Pontocerebellar Hypoplasia, Type 2e
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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