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NPTX2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150863

NPTX2 Antibody: Neuronal pentraxin (NPTX) 2 was initially identified as a member of the pentraxin family having high homology to NPTX1. Unlike NPTX1 however, NPTX2 is expressed in testes, pancreas, liver, heart, skeletal muscle as well as brain. NPTX2… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
NPTX2, Polyclonal Antibody
Full Product Name
NPTX2 Antibody
Product Gene Name
anti-NPTX2 antibody
Product Synonym Names
NPTX2; NP2; NARP; NP-II; Neuronal pentraxin-2; Neuronal pentraxin II; NP2; neuronal pentraxin II
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid; NPTX2 Antibody is supplied in PBS containing 0.02% sodium azide.
Purity/Purification
NPTX2 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
NPTX2 peptide (MBS152742) is used for blocking the activity of NPTX2 antibody (MBS150863)
Molecular Weight
Predicted MW: 47 KD
Observed MW: 47 KD
OMIM
600750
3D Structure
ModBase 3D Structure for P47972
Conjugate
Unconjugated
Immunogen
NPTX2 antibody (MBS150863) was raised against a 16 amino acid synthetic peptide near the center of the human NPTX2.
The immunogen is located within amino acids 170-220 of NPTX2.
Homology
Predicted species reactivity based on immunogen sequence: Guinea pig: (88%)
Validation
Independent Antibody Validation in Cell lines shows similar NPTX2expression profile in human and mouse cell lines detected by two independent anti-NPTX2 antibodies that recognize different epitopes, MBS150863 against central domain. NPTX2 proteins are detected only in HeLa and HepG2 cell lines by the two independent antibodies.
Isoforms
Human NPTX2 has one isoform (431aa, 47kD). Mouse NPTX2 has one isoform (429aa,47.1kD) and Rat NPTX2 also has one isoform (432aa, 47.4kD). 4573 can detect human,mouse and rat.
Positive Control
1) MBS151663 - Mouse Brain Tissue Lysate
2) MBS151603 - HeLa Cell Lysate
3) MBS151605 - HepG2 Cell Lysate
4) MBS154323 - Human Brain Tissue Slide
Applications Tested/Suitable for anti-NPTX2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF)
Application Notes for anti-NPTX2 antibody
WB: 0.5-4 ug/mL
IHC: 5 ug/mL
IF: 20 ug/mL

Antibody validated: Western Blot in human, mouse and rat samples;Immunohistochemistry in human and mouse samples; Immunofluorescence in humanand mouse samples. All other applications and species not yet tested.

Western Blot (WB) of anti-NPTX2 antibody

Loading: 15 ug of lysates per lane. Antibodies: NPTX2 MBS150863 (A: 0.5 ug/mL, B: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at
Rabbit Anti-NPTX2 Polyclonal Antibody – Cat# MBS150863

Immunohistochemistry (IHC) of anti-NPTX2 antibody

Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antibody (MBS150863) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Rabbit Anti-NPTX2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150863

Immunofluorescence (IF) of anti-NPTX2 antibody

Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling NPTX2 with MBS150863 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Rabbit Anti-NPTX2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150863

Immunohistochemistry (IHC) of anti-NPTX2 antibody

Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-NPTX2 antibody (MBS150863) at 5ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Rabbit Anti-NPTX2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150863

Western Blot (WB) of anti-NPTX2 antibody

Western blot analysis of NPTX2 expression in mouse brain tissue lysate with NPTX2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-NPTX2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS150863

Immunofluorescence (IF) of anti-NPTX2 antibody

Immunofluorescence of NPTX2 in mouse brain tissue with NPTX2 antibody at 20 μg/mL.

Red: NPTX2 Antibody (4573)
Blue: DAPI staining
Rabbit Anti-NPTX2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150863

Testing Data of anti-NPTX2 antibody

Loading: 15 ug of lysates per lane. Antibodies: NPTX2 MBS150863(2 ug/mL) and beta-actin MBS150383 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Rabbit Anti-NPTX2 Polyclonal Antibody, Testing Data – Cat# MBS150863

Western Blot (WB) of anti-NPTX2 antibody

Loading: 15 ug of lysates per lane. Antibodies: NPTX2 MBS150863(1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. A: Absence of blocking peptide B: Presence of blocking peptide.
Rabbit Anti-NPTX2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS150863

Immunofluorescence (IF) of anti-NPTX2 antibody

Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with MBS150863 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red) and DAPI staining (blue).
Rabbit Anti-NPTX2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150863

Other Notes

Small volumes of anti-NPTX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NPTX2 purchase

MBS150863 is an antibody targeting NPTX2. Also known as NPTX2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NPTX2. It may not necessarily be applicable to this product.

NCBI GI #
28195384
NCBI GeneID
4885
NCBI Accession #
NP_002514
UniProt Primary Accession #
P47972
UniProt Secondary Accession #
Q86XV7; Q96G70; A4D267
UniProt Related Accession #
P47972
GenBank Nucleotide #
NM_002523.2
NCBI Official Full Name
neuronal pentraxin-2
NCBI Official Synonym Full Names
neuronal pentraxin II
NCBI Official Symbol
NPTX2
NCBI Official Synonym Symbols
NP2; NARP; NP-II
UniProt Protein Name
Neuronal pentraxin-2
UniProt Synonym Protein Names
Neuronal pentraxin II; NP-II
Protein Family
Neuronal pentraxin
UniProt Gene Name
NPTX2
UniProt Synonym Gene Names
NP2; NP-II
UniProt Entry Name
NPTX2_HUMAN
NCBI Protein Information
neuronal pentraxin-2; apexin; neuronal activity-regulated pentaxin

NCBI Summary for NPTX2

This gene encodes a member of the family of neuronal petraxins, synaptic proteins that are related to C-reactive protein. This protein is involved in excitatory synapse formation. It also plays a role in clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. Up-regulation of this gene in Parkinson disease (PD) tissues suggests that the protein may be involved in the pathology of PD. [provided by RefSeq, Feb 2009]
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UniProt Comments for NPTX2

NPTX2: Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q21.3-q22.1

Cellular Component: extracellular region

Molecular Function: metal ion binding; carbohydrate binding

Biological Process: synaptic transmission
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Research Articles on NPTX2

1. NPTX2 repressed NF-kappaB activity by inhibiting AKT through a p53-PTEN-dependent pathway, thus explaining the hypermethylation and downregulation of NPTX2 in NF-kappaB-activated high-risk glioblastomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-NPTX2 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >8 publications with NPTX2 and Adenocarcinoma
 Nervous System Diseases Antibodies  >6 publications with NPTX2 and Nervous System Diseases
 Mental Disorders Antibodies  >4 publications with NPTX2 and Mental Disorders
 Neoplasms, Experimental Antibodies  >2 publications with NPTX2 and Neoplasms, Experimental
 Neurobehavioral Manifestations Antibodies  >1 publications with NPTX2 and Neurobehavioral Manifestations
 Mood Disorders Antibodies  >1 publications with NPTX2 and Mood Disorders
 Movement Disorders Antibodies  >1 publications with NPTX2 and Movement Disorders
 Breast Neoplasms Antibodies  >1 publications with NPTX2 and Breast Neoplasms
 Necrosis Antibodies  >1 publications with NPTX2 and Necrosis
 Edema Antibodies  >1 publications with NPTX2 and Edema

Organs/Tissues associated with anti-NPTX2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with NPTX2 and Brain
 Pancreas Antibodies  >10 publications with NPTX2 and Pancreas
 Embryonic Tissue Antibodies  >2 publications with NPTX2 and Embryonic Tissue
 Kidney Antibodies  >2 publications with NPTX2 and Kidney
 Testis Antibodies  >2 publications with NPTX2 and Testis
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