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GAPDH Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150873

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GAPDH, Polyclonal Antibody
Full Product Name
GAPDH Antibody
Product Gene Name
Product Synonym Names
Glyceraldehyde-3-phosphate dehydrogenase; G3PDH; GAPD; (CT) an enzyme in the glycolytic pathway
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, mouse, rat
Form/Format
Liquid. PBS containing 0.02% sodium azide.
Purity/Purification
Affinity chromatography purified via peptide column
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for three months and -20 degree C. Stable for up to one year.
Antibody/Peptide Pairs
GAPDH peptide (MBS152359) is used for blocking the activity of GAPDH antibody (MBS150873)
Molecular Weight
36,053 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (6643657..6647536). Location: 12p13
OMIM
138400
3D Structure
ModBase 3D Structure for P04406
Immunogen
16 amino acid peptide from near the carboxy terminus of human GAPDH.
Blocking Peptide
MBS152359 GAPDH Peptide
Positive Control 1
MBS151603 HeLa Lysate
Applications Tested/Suitable for anti-GAPDH antibody
ELISA, Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GAPDH antibody
GAPDH antibody can be used for the detection of GAPDH by Western Blot at 0.5 - 1 ug/mL.

Western Blot (WB) of anti-GAPDH antibody

Western blot analysis of GAPDH in A431, Daudi, HepG2, HL60, Jurkat, Human kidney, Mouse lung, and Chicken liver lysate with GAPDH antibody 0.5 μg/mL.
Rabbit Anti-GAPDH Polyclonal Antibody – Cat# MBS150873

Immunohistochemistry (IHC) of anti-GAPDH antibody

Immunohistochemical staining of GAPDH in HeLa cells at a dilution of 10 μg/mL.
Rabbit Anti-GAPDH Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150873

Immunofluorescence (IF) of anti-GAPDH antibody

Immunofluorescence of GAPDH in HeLa cells with GAPDH antibody at 20 μg/mL.
Rabbit Anti-GAPDH Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150873

Product References and Citations for anti-GAPDH antibody

* Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
* Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.

Other Notes

Small volumes of anti-GAPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAPDH purchase

MBS150873 is an antibody targeting GAPDH. Also known as GAPDH Antibody. Reactive in Human, mouse, rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Synonym Protein Names
Peptidyl-cysteine S-nitrosylase GAPDH
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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