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GNPDA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150944

GNPDA1 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA1 is one of two mammalian glucosamine-6-phosphate… More details

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Product Overview

Online Datasheet in PDF
Product Name
GNPDA1, Polyclonal Antibody
Full Product Name
GNPDA1 Antibody
Product Gene Name
Product Synonym Names
GNPDA1; GPI; HLN; GNP1; GNPI; GNPDA; KIAA0060; Glucosamine-6-phosphate isomerase 1; Glucosamine-6-phosphate deaminase 1; GNPDA 1; glucosamine-6-phosphate deaminase 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
GNPDA1 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
GNPDA1 is predicted to not cross-react with GNPDA2.
Preparation and Storage
GNPDA1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
GNPDA1 peptide (MBS153156) is used for blocking the activity of GNPDA1 antibody (MBS150944)
Molecular Weight
24,047 Da
OMIM
601798
3D Structure
ModBase 3D Structure for P46926
Conjugate
Unconjugated
Immunogen
GNPDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GNPDA1.
Buffer
GNPDA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-GNPDA1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-GNPDA1 antibody
GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-GNPDA1 antibody

Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 μg/mL.
Rabbit Anti-GNPDA1 Polyclonal Antibody – Cat# MBS150944

Immunocytochemistry (IHC) of anti-GNPDA1 antibody

Immunocytochemistry of GNPDA1 in 293 cells with GNPDA1 antibody at 5 μg/mL.
Rabbit Anti-GNPDA1 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150944

Immunofluorescence (IF) of anti-GNPDA1 antibody

Immunofluorescence of GNPDA1 in 293 cells with GNPDA1 antibody at 20 μg/mL.
Rabbit Anti-GNPDA1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150944

Other Notes

Small volumes of anti-GNPDA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GNPDA1 purchase

MBS150944 is an antibody targeting GNPDA1. Also known as GNPDA1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GNPDA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
glucosamine-6-phosphate deaminase
NCBI Official Synonym Full Names
glucosamine-6-phosphate deaminase 1
NCBI Official Symbol
GNPDA1
NCBI Official Synonym Symbols
GPI; HLN; GNP1; GNPI; GNPDA
UniProt Protein Name
Glucosamine-6-phosphate isomerase 1
UniProt Synonym Protein Names
Glucosamine-6-phosphate deaminase 1; GNPDA 1; GlcN6P deaminase 1; Oscillin
UniProt Gene Name
GNPDA1
UniProt Synonym Gene Names
GNPI; HLN; KIAA0060; GNPDA 1; GlcN6P deaminase 1
UniProt Entry Name
GNPI1_HUMAN
NCBI Protein Information
glucosamine-6-phosphate isomerase 1; GNPDA 1; oscillin; glcN6P deaminase 1

NCBI Summary for GNPDA1

Glucosamine-6-phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium (Arreola et al., 2003 [PubMed 12965206]).[supplied by OMIM, Jan 2010]

UniProt Comments for GNPDA1

GNPDA1: Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo. Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.

Protein type: EC 3.5.99.6; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Isomerase; Hydrolase

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: cytoplasm

Molecular Function: glucosamine-6-phosphate deaminase activity; hydrolase activity

Biological Process: generation of precursor metabolites and energy; single fertilization; glucosamine catabolic process; carbohydrate metabolic process; N-acetylglucosamine metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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