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SPINK2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151088

SPINK2 Antibody: Human serine proteinase inhibitor Kazal-type 2 (SPINK2) is required for maintaining normal spermatogenesis and potentially regulates serine protease-mediated apoptosis in male germ cells. It contains a typical kazal domain composed by six… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SPINK2, Polyclonal Antibody
Full Product Name
SPINK2 Antibody
Product Gene Name
Product Synonym Names
SPINK2; HUSI-II; Serine protease inhibitor Kazal-type 2; Acrosin-trypsin inhibitor; serine peptidase inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
SPINK2 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
SPINK2 antibody is predicted to not cross-react with other SPINK family members.
Preparation and Storage
SPINK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
SPINK2 peptide (MBS151989) is used for blocking the activity of SPINK2 antibody (MBS151088)
Molecular Weight
Predicted: 15 kDa
Observed: 18 kDa
OMIM
605753
3D Structure
ModBase 3D Structure for P20155
Conjugate
Unconjugated
Immunogen
SPINK2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of mouse SPINK2 . The immunogen is located within the last 50 amino acids of SPINK2..
Buffer
SPINK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Homology
Predicted species based on immunogen sequence : Rat (100%)
Applications Tested/Suitable for anti-SPINK2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Application Notes for anti-SPINK2 antibody
SPINK2 antibody can be used for detection of SPINK2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Optimal dilutions for each application to be determined by the researcher.

Western Blot (WB) of anti-SPINK2 antibody

Western blot analysis of SPINK2 in human testis tissue lysate with SPINK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-SPINK2 Polyclonal Antibody – Cat# MBS151088

Immunohistochemistry (IHC) of anti-SPINK2 antibody

Immunohistochemistry of SPINK2 in mouse testis tissue with SPINK2 antibody at 2.5 μg/mL.
Rabbit Anti-SPINK2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151088

Immunofluorescence (IF) of anti-SPINK2 antibody

Immunofluorescence of SPINK2 in mouse testis tissue with SPINK2 antibody at 20 μg/mL.
Rabbit Anti-SPINK2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151088

Other Notes

Small volumes of anti-SPINK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPINK2 purchase

MBS151088 is an antibody targeting SPINK2. Also known as SPINK2 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPINK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine protease inhibitor Kazal-type 2 isoform 2
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)
NCBI Official Symbol
SPINK2
NCBI Official Synonym Symbols
HUSI-II
UniProt Protein Name
Serine protease inhibitor Kazal-type 2
UniProt Synonym Protein Names
Acrosin-trypsin inhibitor; Epididymis tissue protein Li 172; HUSI-II
UniProt Gene Name
SPINK2
UniProt Entry Name
ISK2_HUMAN
NCBI Protein Information
serine protease inhibitor Kazal-type 2; epididymis tissue protein Li 172

NCBI Summary for SPINK2

This gene encodes a member of the family of serine protease inhibitors of the Kazal type (SPINK). The encoded protein acts as a trypsin and acrosin inhibitor in the genital tract and is localized in the spermatozoa. The protein has been associated with the progression of lymphomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
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UniProt Comments for SPINK2

SPINK2: Strong inhibitor of acrosin in male and/or female genital tract. Also inhibits trypsin.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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