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ATG5 Antibody – Chicken Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151098

ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients… More details

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Product Overview

Online Datasheet in PDF
Product Name
ATG5, Polyclonal Antibody
Full Product Name
ATG5 Antibody
Product Gene Name
anti-ATG5 antibody
Product Synonym Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE; ATG5 autophagy related 5 homolog (S. cerevisiae)
Clonality
Polyclonal
Isotype
IgY
Host
Chicken
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
ATG5 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.
Preparation and Storage
ATG5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
32,447 Da
3D Structure
ModBase 3D Structure for A9UGY9
Conjugate
Unconjugated
Immunogen
ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5.
Buffer
ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-ATG5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ATG5 antibody
ATG5 antibody can be used for the detection of ATG5 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.

Western Blot (WB) of anti-ATG5 antibody

Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines

Loading: 15 μg of lysates per lane.
Antibodies: ATG5 5031 (2 μg/mL), ATG5 64-229 (2 μg/mL), ????-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-chicken or goat anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.
Chicken Anti-ATG5 Polyclonal Antibody – Cat# MBS151098

Western Blot (WB) of anti-ATG5 antibody

Figure 1 Western Blot Validation of ATG5 in Mouse Spleen

Loading: 15 μg of lysates per lane.
Antibodies: ATG5, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.
Lane A: ATG5 5031, 1 μg/mL
Lane B: ATG5 5031, 2 μg/mL
Chicken Anti-ATG5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS151098

Western Blot (WB) of anti-ATG5 antibody

Figure 3 Western Blot Validation of ATG5 in Human Cell Lines

Loading: 15 μg of lysates per lane.
Antibodies: ATG 5031 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.
Chicken Anti-ATG5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS151098

Immunohistochemistry (IHC) of anti-ATG5 antibody

Figure 3 Immunohistochemistry Validation of ATG5 in Human Spleen

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATG5 antibody (5031) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-chicken IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Chicken Anti-ATG5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151098

Other Notes

Small volumes of anti-ATG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG5 purchase

MBS151098 is an antibody targeting ATG5. Also known as ATG5 Antibody. Reactive in Human, Mouse, Rat. Host: Chicken.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG5. It may not necessarily be applicable to this product.

NCBI GI #
119568800
NCBI GeneID
9474
NCBI Accession #
EAW48415
UniProt Primary Accession #
A9UGY9
UniProt Related Accession #
Q9H1Y0
NCBI Official Full Name
ATG5 autophagy related 5 homolog (S. cerevisiae), isoform CRA_b
NCBI Official Synonym Full Names
autophagy related 5
NCBI Official Symbol
ATG5
NCBI Official Synonym Symbols
ASP; APG5; APG5L; hAPG5; APG5-LIKE
UniProt Protein Name
Autophagy protein 5
Protein Family
Autophagy protein
UniProt Gene Name
ATG5
UniProt Entry Name
A9UGY9_HUMAN
NCBI Protein Information
autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog

UniProt Comments for ATG5

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system; Apoptosis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; innate immune response; autophagy; negative regulation of protein ubiquitination; blood vessel remodeling; negative regulation of interferon type I production; otolith development; autophagic vacuole formation; negative regulation of apoptosis
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Research Articles on ATG5

1. Genetic variation in ATG5 is associated with increased susceptibility for thyroid carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ATG5 antibody

 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways antibodies  RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 Shigellosis Pathway antibodies  Shigellosis Pathway Diagram

Diseases associated with anti-ATG5 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >58 publications with ATG5 and Necrosis
 Cardiovascular Diseases Antibodies  >55 publications with ATG5 and Cardiovascular Diseases
 Inflammation Antibodies  >55 publications with ATG5 and Inflammation
 Disease Models, Animal Antibodies  >47 publications with ATG5 and Disease Models, Animal
 Liver Diseases Antibodies  >42 publications with ATG5 and Liver Diseases
 Gastrointestinal Diseases Antibodies  >35 publications with ATG5 and Gastrointestinal Diseases
 Heart Diseases Antibodies  >34 publications with ATG5 and Heart Diseases
 Carcinoma, Hepatocellular Antibodies  >27 publications with ATG5 and Carcinoma, Hepatocellular
 Breast Neoplasms Antibodies  >25 publications with ATG5 and Breast Neoplasms
 Liver Neoplasms Antibodies  >24 publications with ATG5 and Liver Neoplasms

Organs/Tissues associated with anti-ATG5 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >93 publications with ATG5 and Blood
 Brain Antibodies  >89 publications with ATG5 and Brain
 Liver Antibodies  >69 publications with ATG5 and Liver
 Muscle Antibodies  >59 publications with ATG5 and Muscle
 Lung Antibodies  >58 publications with ATG5 and Lung
 Heart Antibodies  >52 publications with ATG5 and Heart
 Kidney Antibodies  >35 publications with ATG5 and Kidney
 Eye Antibodies  >31 publications with ATG5 and Eye
 Bone Antibodies  >31 publications with ATG5 and Bone
 Prostate Antibodies  >25 publications with ATG5 and Prostate
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