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PIG-Y (PIGY) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151115

PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PIG-Y (PIGY), Polyclonal Antibody
Full Product Name
PIG-Y Antibody
Product Gene Name
Product Synonym Names
PIG-Y; PIG-Y; Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y; Phosphatidylinositol-glycan biosynthesis class Y protein; PIG-Y; phosphatidylinositol glycan anchor biosynthesis, class Y
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
PIG-Y Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
This antibody only detects isoform 1 of PIG-Y.
Preparation and Storage
PIG-Y antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
PIG-Y peptide (MBS152108) is used for blocking the activity of PIG-Y antibody (MBS151115)
Molecular Weight
12,655 Da
3D Structure
ModBase 3D Structure for Q3MUY2
Conjugate
Unconjugated
Immunogen
PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PIG-Y.
Buffer
PIG-Y Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-PIGY antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-PIGY antibody
PIG-Y antibody can be used for detection of PIG-Y by Western blot at 2 mug/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-PIGY antibody

Western blot analysis of PIG-Y in human skeletal muscle tissue lysate with PIG-Y antibody at 5 μg/mL.
Rabbit Anti-PIGY Polyclonal Antibody – Cat# MBS151115

Immunohistochemistry (IHC) of anti-PIGY antibody

Immunohistochemistry of PIGY in human spleen tissue with PIGY antibody at 2.5 μg/mL.
Rabbit Anti-PIGY Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151115

Immunofluorescence (IF) of anti-PIGY antibody

Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 μg/mL.
Rabbit Anti-PIGY Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151115

Other Notes

Small volumes of anti-PIGY antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIGY purchase

MBS151115 is an antibody targeting PIGY. Also known as PIG-Y Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PIGY. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein preY, mitochondrial
NCBI Official Synonym Full Names
PIGY upstream reading frame
NCBI Official Symbol
PYURF
NCBI Official Synonym Symbols
PREY
UniProt Protein Name
Protein preY, mitochondrial
UniProt Synonym Protein Names
PIGY upstream reading frame protein
UniProt Gene Name
PYURF
UniProt Synonym Gene Names
PREY
UniProt Entry Name
PREY_HUMAN
NCBI Protein Information
protein preY, mitochondrial; PIGY upstream reading frame protein

NCBI Summary for PIGY

The product of this gene, which is well-conserved, is encoded by the same bicistronic transcript that encodes phosphatidylinositol glycan anchor biosynthesis, class Y, but the two proteins are unrelated. This gene represents the protein encoded by the upstream open reading frame, while the protein encoded by the downstream open reading frame is represented by GeneID:84992. [provided by RefSeq, Aug 2012]
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UniProt Comments for PIGY

PIGY: Component of the GPI-GlcNAc transferase (GPI-GnT) complex in the endoplasmic reticulum, a complex that catalyzes transfer of GlcNAc from UDP-GlcNAc to an acceptor phosphatidylinositol, the first step in the production of GPI-anchors for cell surface proteins. May act by regulating the catalytic subunit PIGA. Belongs to the PREY family.

Protein type: Membrane protein, multi-pass; Endoplasmic reticulum; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; endoplasmic reticulum membrane; integral to membrane; plasma membrane

Biological Process: GPI anchor biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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