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DFF40 (DFFB) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151183

DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DFF40 (Dffb), Polyclonal Antibody
Full Product Name
DFF40 Antibody
Product Gene Name
anti-Dffb antibody
Product Synonym Names
DFF40; CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik; Cad; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; CAD; DNA fragmentation factor, beta subunit
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
DFF40 Antibody is affinity chromatography purified via peptide column.
Preparation and Storage
DFF40 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
DFF40 peptide (MBS152180) is used for blocking the activity of DFF40 antibody (MBS151183)
Molecular Weight
40 kDa
3D Structure
ModBase 3D Structure for O54788
Conjugate
Unconjugated
Immunogen
DFF40 antibody was raised against a 16 amino acid peptide near the center of human DFF40.
Buffer
DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-Dffb antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-Dffb antibody
DFF40 antibody can be used for detection of DFF40 /CAD by Western blot at 0.5 - 2 mug/mL. dilution. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.

Western Blot (WB) of anti-Dffb antibody

Western blot analysis of DFF40 in (A) K562 and (B) and Jurkat cell lysate with DFF40 antibody at 1 μg/mL.
Rabbit Anti-DFFB Polyclonal Antibody – Cat# MBS151183

Immunocytochemistry (IHC) of anti-Dffb antibody

Immunocytochemistry of DFF40 in K562 cells with DFF40 antibody at 10 μg/mL.
Rabbit Anti-DFFB Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS151183

Other Notes

Small volumes of anti-Dffb antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Dffb purchase

MBS151183 is an antibody targeting Dffb. Also known as DFF40 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Dffb. It may not necessarily be applicable to this product.

NCBI GI #
160948620
NCBI GeneID
13368
NCBI Accession #
NP_031885
UniProt Primary Accession #
O54788
UniProt Related Accession #
O54788
GenBank Nucleotide #
NM_007859.4
NCBI Official Full Name
DNA fragmentation factor subunit beta
NCBI Official Synonym Full Names
DNA fragmentation factor, beta subunit
NCBI Official Symbol
Dffb
NCBI Official Synonym Symbols
CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik
UniProt Protein Name
DNA fragmentation factor subunit beta
UniProt Synonym Protein Names
Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; DNA fragmentation factor 40 kDa subunit; DFF-40
Protein Family
DNA fragmentation factor
UniProt Gene Name
Dffb
UniProt Synonym Gene Names
Cad; CAD; Caspase-activated DNase; DFF-40
UniProt Entry Name
DFFB_MOUSE
NCBI Protein Information
DNA fragmentation factor subunit beta; DFF-40; caspase-activated DNase; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; DNase inhibited by DNA fragmentation factor; DNA fragmentation factor, 40 kD, beta subunit

UniProt Comments for Dffb

DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Deoxyribonuclease; EC 3.-.-.-

Cellular Component: cytoplasm; nuclear chromatin; nucleolus; intracellular; nucleus; cytosol

Molecular Function: protein binding; nuclease activity; enzyme binding; DNA binding; hydrolase activity; deoxyribonuclease activity; GPI-anchor transamidase activity

Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation
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Research Articles on Dffb

1. the highest order of chromatin compaction observed in the later steps of caspase-dependent apoptosis relies on DFF40/CAD-mediated DNA damage by generating 3'-OH ends in single-strand rather than double-strand DNA nicks/breaks

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Dffb antibody

 Products by Pathway  Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptosis Signaling Pathway antibodies  Apoptosis Signaling Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Programmed Cell Death Pathway antibodies  Programmed Cell Death Pathway Diagram

Diseases associated with anti-Dffb antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >13 publications with Dffb and Necrosis
 Carcinoma Antibodies  >7 publications with Dffb and Carcinoma
 Adenocarcinoma Antibodies  >4 publications with Dffb and Adenocarcinoma
 Immune System Diseases Antibodies  >4 publications with Dffb and Immune System Diseases
 Neoplasms, Experimental Antibodies  >4 publications with Dffb and Neoplasms, Experimental
 Cognition Disorders Antibodies  >3 publications with Dffb and Cognition Disorders
 Cardiovascular Diseases Antibodies  >3 publications with Dffb and Cardiovascular Diseases
 Inflammation Antibodies  >2 publications with Dffb and Inflammation
 Disease Models, Animal Antibodies  >2 publications with Dffb and Disease Models, Animal
 Lung Neoplasms Antibodies  >1 publications with Dffb and Lung Neoplasms

Organs/Tissues associated with anti-Dffb antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with Dffb and Brain
 Lung Antibodies  >3 publications with Dffb and Lung
 Kidney Antibodies  >2 publications with Dffb and Kidney
 Spinal Cord Antibodies  >2 publications with Dffb and Spinal Cord
 Skin Antibodies  >1 publications with Dffb and Skin
 Testis Antibodies  >1 publications with Dffb and Testis
 Bone Marrow Antibodies  >1 publications with Dffb and Bone Marrow
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