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RABGEF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151191

RABEX5 Antibody: RABEX5 is a guanine nucleotide exchange factor (GEF) that forms a complex with rabaptin-5 and specifically activates Rab5, a small GTPase that plays an essential role in endocytic traffic. RABEX-5 is thought to also interact with another… More details

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Product Overview

Online Datasheet in PDF
Product Name
RABEX5 (RABGEF1), Polyclonal Antibody
Full Product Name
RABEX5 Antibody
Product Gene Name
anti-RABGEF1 antibody
Product Synonym Names
RABEX5; RAP1; RABEX5; rabex-5; RAP1; RAB guanine nucleotide exchange factor (GEF) 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
RABEX5 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
At least four isoforms of RABEX5 are known to exist; this antibody will recognize all four. RABEX5 antibody is predicted to not cross-react with other LRRFIP family members.
Preparation and Storage
RABEX5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
RABEX5 peptide (MBS151811) is used for blocking the activity of RABEX5 antibody (MBS151191)
Molecular Weight
58,526 Da
OMIM
609700
3D Structure
ModBase 3D Structure for Q9UJ41
Conjugate
Unconjugated
Immunogen
RABEX5 antibody was raised against a 19 amino acid synthetic peptide near the center terminus of human RABEX5.
Buffer
RABEX5 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-RABGEF1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-RABGEF1 antibody
RABEX5 antibody can be used for detection of RABEX5 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-RABGEF1 antibody

Western blot analysis of RABEX5 in human liver tissue lysate with RABEX5 antibody at (A) 1 and (B) 2 μg/mL.
Rabbit Anti-RABGEF1 Polyclonal Antibody – Cat# MBS151191

Immunohistochemistry (IHC) of anti-RABGEF1 antibody

Immunohistochemistry of RABEX5 in mouse brain tissue with RABEX5 antibody at 5 μg/mL.
Rabbit Anti-RABGEF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151191

Immunofluorescence (IF) of anti-RABGEF1 antibody

Immunofluorescence of RABEX5 in mouse brain tissue with RABEX5 antibody at 20 μg/mL.
Rabbit Anti-RABGEF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151191

Other Notes

Small volumes of anti-RABGEF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RABGEF1 purchase

MBS151191 is an antibody targeting RABGEF1. Also known as RABEX5 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RABGEF1. It may not necessarily be applicable to this product.

NCBI GI #
7657496
NCBI GeneID
27342
NCBI Accession #
NP_055319
UniProt Primary Accession #
Q9UJ41
UniProt Secondary Accession #
Q3HKR2; Q3HKR3; Q53FG0; B4DZM7
UniProt Related Accession #
Q9UJ41
GenBank Nucleotide #
NM_014504.2
NCBI Official Full Name
rab5 GDP/GTP exchange factor isoform c
NCBI Official Synonym Full Names
RAB guanine nucleotide exchange factor (GEF) 1
NCBI Official Symbol
RABGEF1
NCBI Official Synonym Symbols
RAP1; RABEX5; rabex-5
UniProt Protein Name
Rab5 GDP/GTP exchange factor
UniProt Synonym Protein Names
RAP1; Rabaptin-5-associated exchange factor for Rab5; Rabex-5
Protein Family
Rab5 GDP/GTP exchange factor
UniProt Gene Name
RABGEF1
UniProt Synonym Gene Names
RABEX5
UniProt Entry Name
RABX5_HUMAN
NCBI Protein Information
rab5 GDP/GTP exchange factor; rabaptin-5-associated exchange factor for Rab5

NCBI Summary for RABGEF1

RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM, Mar 2010]

UniProt Comments for RABGEF1

RABGEF1: Rab effector protein acting as linker between gamma- adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase. Interacts with RGS14; the interaction is GTP-dependent. Heterodimer with RABEP1. The heterodimer binds RAB4A and RAB5A that have been activated by GTP-binding. Interacts with RAB21, and with 100-fold lower affinity also with RAB22. Binds TSC2, GGA1, GGA2, GGA3, AP1G1 and AP1G2. Interacts with ubiquitinated EGFR. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; GAPs, Rab

Chromosomal Location of Human Ortholog: 7q11.21

Cellular Component: recycling endosome; early endosome; vesicle

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; Rab guanyl-nucleotide exchange factor activity; Rab GTPase binding

Biological Process: negative regulation of protein amino acid phosphorylation; negative regulation of mast cell degranulation; negative regulation of inflammatory response; protein ubiquitination; negative regulation of Ras protein signal transduction; endocytosis; negative regulation of receptor-mediated endocytosis; protein targeting to membrane; negative regulation of leukocyte migration
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Research Articles on RABGEF1

1. RABEX-5 mRNA expression is a strong predictor of poor prognosis in prostate cancer patients treated by radical prostatectomy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RABGEF1 antibody

 Reference Product  PubMed Publications
 RABEP1 antibody  >7 publications with RABGEF1 and RABEP1
 EEA1 antibody  >2 publications with RABGEF1 and EEA1
 RAB5A antibody  >2 publications with RABGEF1 and RAB5A
 RABEP2 antibody  >1 publications with RABGEF1 and RABEP2
 GGA1 antibody  >1 publications with RABGEF1 and GGA1
 RAB21 antibody  >1 publications with RABGEF1 and RAB21

Diseases associated with anti-RABGEF1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >6 publications with RABGEF1 and Inflammation
 Weight Loss Antibodies  >1 publications with RABGEF1 and Weight Loss
 Kidney Diseases Antibodies  >1 publications with RABGEF1 and Kidney Diseases
 Hyperplasia Antibodies  >1 publications with RABGEF1 and Hyperplasia

Organs/Tissues associated with anti-RABGEF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with RABGEF1 and Brain
 Blood Antibodies  >5 publications with RABGEF1 and Blood
 Skin Antibodies  >4 publications with RABGEF1 and Skin
 Lymph Antibodies  >3 publications with RABGEF1 and Lymph
 Lymph Node Antibodies  >3 publications with RABGEF1 and Lymph Node
 Bone Antibodies  >2 publications with RABGEF1 and Bone
 Stomach Antibodies  >1 publications with RABGEF1 and Stomach
 Thyroid Antibodies  >1 publications with RABGEF1 and Thyroid
 Spleen Antibodies  >1 publications with RABGEF1 and Spleen
 Prostate Antibodies  >1 publications with RABGEF1 and Prostate
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