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PDCD1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151192

PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PD-1 (PDCD1), Polyclonal Antibody
Full Product Name
PD-1 Antibody
Product Gene Name
Product Synonym Names
PD-1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; PD1; Programmed cell death protein 1; Protein PD-1; PDCD1; PDCD-1; programmed cell death 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
PD-1 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
PD-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
PD-1 peptide (MBS152609) is used for blocking the activity of PD-1 antibody (MBS151192)
Molecular Weight
Predicted: 32 kDa

Observed: 45 kDa
OMIM
gene 605218
3D Structure
ModBase 3D Structure for Q15116
Conjugate
Unconjugated
Immunogen
PD-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human PD-1.
Buffer
PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-PDCD1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-PDCD1 antibody
PD-1 antibody can be used for detection of PD-1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-PDCD1 antibody

Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Rabbit Anti-PDCD1 Polyclonal Antibody – Cat# MBS151192

Immunohistochemistry (IHC) of anti-PDCD1 antibody

Immunohistochemistry of PD-1 in human tonsil tissue with PD-1 antibody at 5 μg/mL.
Rabbit Anti-PDCD1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151192

Immunofluorescence (IF) of anti-PDCD1 antibody

Immunofluorescence of PD-1 in Human Brain tissue with PD-1 antibody at 20 μg/mL.
Rabbit Anti-PDCD1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151192

Immunohistochemistry (IHC) of anti-PDCD1 antibody

Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 2.5 μg/mL.
Rabbit Anti-PDCD1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151192

Western Blot (WB) of anti-PDCD1 antibody

Western blot analysis of PD-1 in rat thymus cell lysate with PD-1 antibody at 1 μg/mL.
Rabbit Anti-PDCD1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS151192

Other Notes

Small volumes of anti-PDCD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDCD1 purchase

MBS151192 is an antibody targeting PDCD1. Also known as PD-1 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PDCD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
PDCD1
NCBI Official Synonym Symbols
PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
UniProt Protein Name
Programmed cell death protein 1
UniProt Synonym Protein Names
CD_antigen: CD279
UniProt Gene Name
PDCD1
UniProt Synonym Gene Names
PD1; Protein PD-1; hPD-1
UniProt Entry Name
PDCD1_HUMAN
NCBI Protein Information
programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2

NCBI Summary for PDCD1

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
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UniProt Comments for PDCD1

PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; signal transducer activity

Biological Process: apoptosis; multicellular organismal development; T cell costimulation; signal transduction; humoral immune response

Disease: Systemic Lupus Erythematosus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-PDCD1 antibody

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