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PRICKLE2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151254

PRICKLE2 Antibody: PRICKLE2, also known as Pk2 or EPM5, is a member of a highly conserved protein family that function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity. Both PRICKLE2 and the… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
PRICKLE2, Polyclonal Antibody
Full Product Name
PRICKLE2 Antibody
Product Gene Name
Product Synonym Names
PRICKLE2; EPM5; Prickle-like protein 2; prickle homolog 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
PRICKLE2 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
Two isoforms of PRICKLE2 are known to exist. PRICKLE2 antibody is predicted to not cross-react with other PRICKLE protein family members.
Preparation and Storage
PRICKLE2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
PRICKLE2 peptide (MBS152444) is used for blocking the activity of PRICKLE2 antibody (MBS151254)
Molecular Weight
95,615 Da
OMIM
gene 613832
3D Structure
ModBase 3D Structure for Q7Z3G6
Conjugate
Unconjugated
Immunogen
PRICKLE2 antibody was raised against a 15 amino acid synthetic peptide near the center of human PRICKLE2.
Buffer
PRICKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-PRICKLE2 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-PRICKLE2 antibody
PRICKLE2 antibody can be used for detection of PRICKLE2 by Western blot at 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-PRICKLE2 antibody

Western blot analysis of PRICKLE2 in A-20 lysate with PRICKLE2 antibody at 1 μg/mL.
Rabbit Anti-PRICKLE2 Polyclonal Antibody – Cat# MBS151254

Immunofluorescence (IF) of anti-PRICKLE2 antibody

Immunofluorescence of PRICKLE2 in rat brain cells with PRICKLE2 antibody at 20 μg/mL.
Rabbit Anti-PRICKLE2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151254

Other Notes

Small volumes of anti-PRICKLE2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRICKLE2 purchase

MBS151254 is an antibody targeting PRICKLE2. Also known as PRICKLE2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PRICKLE2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
prickle-like protein 2
NCBI Official Synonym Full Names
prickle homolog 2 (Drosophila)
NCBI Official Symbol
PRICKLE2
NCBI Official Synonym Symbols
EPM5
UniProt Protein Name
Prickle-like protein 2
Protein Family
UniProt Gene Name
PRICKLE2
UniProt Entry Name
PRIC2_HUMAN
NCBI Protein Information
prickle-like protein 2

NCBI Summary for PRICKLE2

This gene encodes a homolog of Drosophila prickle. The exact function of this gene is not known, however, studies in mice suggest that it may be involved in seizure prevention. Mutations in this gene are associated with progressive myoclonic epilepsy type 5. [provided by RefSeq, Dec 2011]

UniProt Comments for PRICKLE2

PRICKLE2: Defects in PRICKLE2 are the cause of progressive myoclonic epilepsy type 5 (EPM5). EPM5 is a neurodegenerative disorder characterized by myoclonic seizures and variable neurologic symptoms including cognitive decline and persistent movement abnormalities. Belongs to the prickle / espinas / testin family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: nuclear membrane; apicolateral plasma membrane; cytoplasm; lateral plasma membrane

Molecular Function: zinc ion binding

Biological Process: establishment and/or maintenance of epithelial cell polarity; neurite development

Disease: Epilepsy, Progressive Myoclonic 5
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PRICKLE2 antibody

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