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MYOZAP (GCOM1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151319

MYOZAP Antibody: MYOZAP, also known as GRINL1A, is a 54 kDa highly conserved cardiac protein. It is strongly expressed in the heart and lung and is a novel component of intercalated disc. MYOZAP interacts with myosin phosphatase-RhoA interacting protein… More details

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Product Overview

Online Datasheet in PDF
Product Name
MYOZAP (GCOM1), Polyclonal Antibody
Full Product Name
MYOZAP Antibody
Product Gene Name
Product Synonym Names
MYOZAP; gcom; Gcom2; MYZAP; GRINL1A; MYZAP-POLR2M; MYOZAP; Myocardial zonula adherens protein; GRINL1A upstream protein; Gup; GRINL1A complex locus
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
MYOZAP Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
Multiple isoforms of MYOZAP are known to exist.
Preparation and Storage
MYOZAP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
MYOZAP peptide (MBS151946) is used for blocking the activity of MYOZAP antibody (MBS151319)
Molecular Weight
64,046 Da
OMIM
614071
3D Structure
ModBase 3D Structure for P0CAP1
Conjugate
Unconjugated
Immunogen
MYOZAP antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human MYOZAP.
Buffer
MYOZAP Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-GCOM1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-GCOM1 antibody
MYOZAP antibody can be used for detection of MYOZAP by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-GCOM1 antibody

Western blot analysis of MYOZAP in rat kidney tissue lysate with MYOZAP antibody at (A) 1 and (B) 2 μg/mL.
Rabbit Anti-GCOM1 Polyclonal Antibody – Cat# MBS151319

Immunohistochemistry (IHC) of anti-GCOM1 antibody

Immunohistochemistry of MYOZAP in human lung tissue with MYOZAP antibody at 5 μg/mL.
Rabbit Anti-GCOM1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151319

Immunofluorescence (IF) of anti-GCOM1 antibody

Immunofluorescence of MYOZAP in human lung tissue with MYOZAP antibody at 20 μg/mL.
Rabbit Anti-GCOM1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151319

Other Notes

Small volumes of anti-GCOM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GCOM1 purchase

MBS151319 is an antibody targeting GCOM1. Also known as MYOZAP Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GCOM1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
GRINL1A combined protein isoform 1
NCBI Official Synonym Full Names
GRINL1A complex locus 1
NCBI Official Symbol
GCOM1
NCBI Official Synonym Symbols
gcom; Gcom2; MYZAP; GRINL1A; MYZAP-POLR2M
UniProt Protein Name
Myocardial zonula adherens protein
UniProt Synonym Protein Names
GRINL1A upstream protein; Gup
UniProt Gene Name
MYZAP
UniProt Synonym Gene Names
MYOZAP; Gup
UniProt Entry Name
MYZAP_HUMAN
NCBI Protein Information
GRINL1A combined protein; MYZAP-POLR2M protein; MYZAP-POLR2M readthrough; GRINL1A combined protein Gcom12; NMDAR1 subunit-interacting protein; myocardial zonula adherens protein; glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A combined protein

NCBI Summary for GCOM1

This locus represents naturally occurring readthrough transcription between the neighboring MYZAP (myocardial zonula adherens protein) and POLR2M (polymerase (RNA) II (DNA directed) polypeptide M) genes on chromosome 15. Alternative splicing results in multiple readthrough transcript variants. Readthrough variants may encode proteins that share sequence identity with the upstream gene product or with both the upstream and downstream gene products. Some readthrough transcript variants are also expected to be candidates for nonsense-mediated decay (NMD). [provided by RefSeq, Oct 2013]
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UniProt Comments for GCOM1

GCOM1: Plays a role in cellular signaling via Rho-related GTP- binding proteins and subsequent activation of transcription factor SRF. Targets TJP1 to cell junctions. In cortical neurons, may play a role in glutaminergic signal transduction through interaction with the NMDA receptor subunit GRIN1. Belongs to the MYZAP family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 15q21.3
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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