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HINT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151397

HINT1 Antibody: Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HINT1, Polyclonal Antibody
Full Product Name
HINT1 Antibody
Product Gene Name
Product Synonym Names
HINT1; HINT; NMAN; PKCI-1; PRKCNH1; HINT; PKCI1; Histidine triad nucleotide-binding protein 1; Protein kinase C inhibitor 1; histidine triad nucleotide binding protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
HINT1 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
HINT1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
HINT1 peptide (MBS152660) is used for blocking the activity of HINT1 antibody (MBS151397)
Molecular Weight
13,802 Da
OMIM
phenotype 601314
3D Structure
ModBase 3D Structure for P49773
Conjugate
Unconjugated
Immunogen
HINT1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human HINT1.
Buffer
HINT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-HINT1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-HINT1 antibody
HINT1 antibody can be used for detection of HINT1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL.

Western Blot (WB) of anti-HINT1 antibody

Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 μg/mL.
Rabbit Anti-HINT1 Polyclonal Antibody – Cat# MBS151397

Immunocytochemistry (IHC) of anti-HINT1 antibody

Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 μg/mL.
Rabbit Anti-HINT1 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS151397

Other Notes

Small volumes of anti-HINT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HINT1 purchase

MBS151397 is an antibody targeting HINT1. Also known as HINT1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HINT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histidine triad nucleotide-binding protein 1
NCBI Official Synonym Full Names
histidine triad nucleotide binding protein 1
NCBI Official Symbol
HINT1
NCBI Official Synonym Symbols
HINT; NMAN; PKCI-1; PRKCNH1
UniProt Protein Name
Histidine triad nucleotide-binding protein 1
UniProt Synonym Protein Names
Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; PKCI-1
Protein Family
UniProt Gene Name
HINT1
UniProt Synonym Gene Names
HINT; PKCI1; PRKCNH1; PKCI-1
UniProt Entry Name
HINT1_HUMAN
NCBI Protein Information
histidine triad nucleotide-binding protein 1; protein kinase C inhibitor 1; adenosine 5'-monophosphoramidase; protein kinase C-interacting protein 1

NCBI Summary for HINT1

The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. This gene has been found to be a tumor suppressing gene. Several transcript variants, but only one of them protein-coding, have been found for this gene. [provided by RefSeq, Dec 2012]
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UniProt Comments for HINT1

HINT1: Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha- acetyl lysine methyl ester and AMP-NH2. Belongs to the HINT family.

Protein type: Hydrolase; Cytoskeletal; EC 3.-.-.-

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytoskeleton; cytoplasm; histone deacetylase complex; plasma membrane; nucleus

Molecular Function: protein kinase C binding; hydrolase activity; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; purine ribonucleotide catabolic process; signal transduction

Disease: Neuromyotonia And Axonal Neuropathy, Autosomal Recessive
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HINT1 antibody

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