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HIGD1A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151415

HIG1 Antibody: HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG1 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIG1 (HIGD1A), Polyclonal Antibody
Full Product Name
HIG1 Antibody
Product Gene Name
Product Synonym Names
HIG1; HIG1; RCF1a; HIG1; HSPC010; HIG1 domain family member 1A; mitochondrial; Hypoxia-inducible gene 1 protein; HIG1 hypoxia inducible domain family, member 1A
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
HIG1 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
HIG1 antibody is predicted to not cross-react with HIG2
Preparation and Storage
HIG1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
HIG1 peptide (MBS152712) is used for blocking the activity of HIG1 antibody (MBS151415)
Molecular Weight
11,770 Da
3D Structure
ModBase 3D Structure for Q9Y241
Conjugate
Unconjugated
Immunogen
HIG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HIG1.
Buffer
HIG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-HIGD1A antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-HIGD1A antibody
HIG1 antibody can be used for detection of HIG1 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL. For immunofluorescence start at 10 mug/mL.

Western Blot (WB) of anti-HIGD1A antibody

Western blot analysis of HIG1 in 293 cell lysate with HIG1 antibody at at (A) 0.5 and (B) 1 μg/mL.
Rabbit Anti-HIGD1A Polyclonal Antibody – Cat# MBS151415

Immunocytochemistry (IHC) of anti-HIGD1A antibody

Immunocytochemistry of HIG1 in 293 cells with HIG1 antibody at 2.5 μg/mL.
Rabbit Anti-HIGD1A Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS151415

Immunofluorescence (IF) of anti-HIGD1A antibody

Immunofluorescence of HIG1 in 293 cells tissue with HIG1 antibody at 10 μg/mL.
Rabbit Anti-HIGD1A Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151415

Other Notes

Small volumes of anti-HIGD1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIGD1A purchase

MBS151415 is an antibody targeting HIGD1A. Also known as HIG1 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HIGD1A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
HIG1 domain family member 1A, mitochondrial isoform a
NCBI Official Synonym Full Names
HIG1 hypoxia inducible domain family, member 1A
NCBI Official Symbol
HIGD1A
NCBI Official Synonym Symbols
HIG1; RCF1a
UniProt Protein Name
HIG1 domain family member 1A, mitochondrial
UniProt Synonym Protein Names
Hypoxia-inducible gene 1 protein; RCF1 homolog A; RCF1a
Protein Family
UniProt Gene Name
HIGD1A
UniProt Synonym Gene Names
HIG1; RCF1a
UniProt Entry Name
HIG1A_HUMAN
NCBI Protein Information
HIG1 domain family member 1A, mitochondrial; RCF1 homolog A; hypoxia inducible gene 1; HIG1 domain family, member 1A; hypoxia-inducible gene 1 protein

UniProt Comments for HIGD1A

HIGD1A: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: protein complex; mitochondrial inner membrane; integral to membrane

Biological Process: cellular response to glucose starvation; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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