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SCUBE1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151427

SCUBE1 Antibody: SCUBE1 is an EGF-related protein with a CUB (C1s-like) domain that is expressed primarily in the developing gonad, nervous system, somites, surface ectoderm and limb buds. The N-terminal EGF-like repeats of SCUBE1 participate in the formation… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SCUBE1, Polyclonal Antibody
Full Product Name
SCUBE1 Antibody
Product Gene Name
Product Synonym Names
SCUBE1; Signal peptide; CUB and EGF-like domain-containing protein 1; signal peptide, CUB domain, EGF-like 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
SCUBE1 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
SCUBE1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
SCUBE1 peptide (MBS153049) is used for blocking the activity of SCUBE1 antibody (MBS151427)
Molecular Weight
107,910 Da
OMIM
611746
3D Structure
ModBase 3D Structure for Q8IWY4
Conjugate
Unconjugated
Immunogen
SCUBE1 antibody was raised against a 14 amino acid synthetic peptide near the center of human SCUBE1.
Buffer
SCUBE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Homology
Predicted species reactivity based on immunogen sequence: Mouse (93%)
Positive control
Daudi Cell Lysate (Catalog Number: MBS151632)
Applications Tested/Suitable for anti-SCUBE1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-SCUBE1 antibody
SCUBE1 antibody can be used for detection of SCUBE1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 20 mug/mL. For immunofluorescence start at 20 ug/mL.
Optimal dilutions for each application to be determined by the researcher.

Western Blot (WB) of anti-SCUBE1 antibody

Western blot analysis of SCUBE1 in Daudi cell lysate with SCUBE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-SCUBE1 Polyclonal Antibody – Cat# MBS151427

Immunocytochemistry (IHC) of anti-SCUBE1 antibody

Immunocytochemistry of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.
Rabbit Anti-SCUBE1 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS151427

Immunofluorescence (IF) of anti-SCUBE1 antibody

Immunofluorescence of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.
Rabbit Anti-SCUBE1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151427

Other Notes

Small volumes of anti-SCUBE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SCUBE1 purchase

MBS151427 is an antibody targeting SCUBE1. Also known as SCUBE1 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SCUBE1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
signal peptide, CUB and EGF-like domain-containing protein 1
NCBI Official Synonym Full Names
signal peptide, CUB domain, EGF-like 1
NCBI Official Symbol
SCUBE1
UniProt Protein Name
Signal peptide, CUB and EGF-like domain-containing protein 1
UniProt Gene Name
SCUBE1
UniProt Entry Name
SCUB1_HUMAN
NCBI Protein Information
signal peptide, CUB and EGF-like domain-containing protein 1; signal peptide-CUB domain-EGF-related 1

NCBI Summary for SCUBE1

This gene encodes a cell surface glycoprotein that is a member of the SCUBE (signal peptide, CUB domain, EGF (epidermal growth factor)-like protein) family. Family members have an amino-terminal signal peptide, nine copies of EGF-like repeats and a CUB domain at the carboxyl terminus. This protein is expressed in platelets and endothelial cells and may play an important role in vascular biology. [provided by RefSeq, Oct 2011]
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UniProt Comments for SCUBE1

SCUBE1: Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: extrinsic to plasma membrane; extracellular space; cell surface; external side of plasma membrane

Molecular Function: identical protein binding; protein heterodimerization activity; calcium ion binding

Biological Process: adult heart development; endothelial cell differentiation; blood coagulation; inflammatory response; protein homooligomerization; post-embryonic development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SCUBE1 antibody

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