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ATG12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151529

ATG12 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATG12, Polyclonal Antibody
Full Product Name
ATG12 Antibody
Product Gene Name
anti-ATG12 antibody
Product Synonym Names
ATG12; APG12; FBR93; APG12L; HAPG12; APG12; Ubiquitin-like protein ATG12; Autophagy-related protein 12; APG12-like; ATG12 autophagy related 12 homolog (S. cerevisiae)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
ATG12 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
ATG12 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
ATG12 peptide (MBS151832) is used for blocking the activity of ATG12 antibody (MBS151529)
Molecular Weight
7,746 Da
OMIM
609608
3D Structure
ModBase 3D Structure for O94817
Conjugate
Unconjugated
Immunogen
ATG12 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG12.
Buffer
ATG12 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-ATG12 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ATG12 antibody
ATG12 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-ATG12 antibody

Western blot analysis of ATG12 in human brain tissue lysate with ATG12 antibody at (A) 0.5, and (B) 1 μg/mL.
Rabbit Anti-ATG12 Polyclonal Antibody – Cat# MBS151529

Immunohistochemistry (IHC) of anti-ATG12 antibody

Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 μg/mL.
Rabbit Anti-ATG12 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151529

Immunofluorescence (IF) of anti-ATG12 antibody

Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 μg/mL.
Rabbit Anti-ATG12 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151529

Other Notes

Small volumes of anti-ATG12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG12 purchase

MBS151529 is an antibody targeting ATG12. Also known as ATG12 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG12. It may not necessarily be applicable to this product.

NCBI GI #
119569340
NCBI GeneID
9140
NCBI Accession #
EAW48955
UniProt Primary Accession #
O94817
UniProt Secondary Accession #
Q6PJV2
UniProt Related Accession #
O94817
NCBI Official Full Name
ATG12 autophagy related 12 homolog (S. cerevisiae), isoform CRA_b
NCBI Official Synonym Full Names
autophagy related 12
NCBI Official Symbol
ATG12
NCBI Official Synonym Symbols
APG12; FBR93; APG12L; HAPG12
UniProt Protein Name
Ubiquitin-like protein ATG12
UniProt Synonym Protein Names
Autophagy-related protein 12; APG12-like
Protein Family
Autophagy protein
UniProt Gene Name
ATG12
UniProt Synonym Gene Names
APG12; APG12L; APG12-like
UniProt Entry Name
ATG12_HUMAN
NCBI Protein Information
ubiquitin-like protein ATG12; Apg12 (autophagy, yeast) homolog; ATG12 autophagy related 12 homolog

NCBI Summary for ATG12

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
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UniProt Comments for ATG12

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: pre-autophagosomal structure membrane

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; cellular response to nitrogen starvation; autophagic vacuole formation
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Research Articles on ATG12

1. Cav-1 competitively interacts with the ATG12-ATG5 system to suppress the formation and function of the latter in lung epithelial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATG12 antibody

 Reference Product  PubMed Publications
 ATG5 antibody  >223 publications with ATG12 and ATG5
 ATG7 antibody  >86 publications with ATG12 and ATG7
 ATG3 antibody  >44 publications with ATG12 and ATG3
 BECN1 antibody  >38 publications with ATG12 and BECN1
 ATG16L1 antibody  >35 publications with ATG12 and ATG16L1
 ATG10 antibody  >31 publications with ATG12 and ATG10
 TECPR1 antibody  >4 publications with ATG12 and TECPR1
 GAPDH antibody  >4 publications with ATG12 and GAPDH
 DDX58 antibody  >4 publications with ATG12 and DDX58
 MAVS antibody  >2 publications with ATG12 and MAVS

Pathways associated with anti-ATG12 antibody

 Products by Pathway  Pathway Diagram
 FoxO Signaling Pathway antibodies  FoxO Signaling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways antibodies  RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram

Diseases associated with anti-ATG12 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >29 publications with ATG12 and Nervous System Diseases
 Inflammation Antibodies  >11 publications with ATG12 and Inflammation
 Breast Neoplasms Antibodies  >10 publications with ATG12 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >10 publications with ATG12 and Cardiovascular Diseases
 Necrosis Antibodies  >9 publications with ATG12 and Necrosis
 Liver Diseases Antibodies  >6 publications with ATG12 and Liver Diseases
 Heart Diseases Antibodies  >5 publications with ATG12 and Heart Diseases
 Muscular Diseases Antibodies  >5 publications with ATG12 and Muscular Diseases
 Carcinoma, Hepatocellular Antibodies  >5 publications with ATG12 and Carcinoma, Hepatocellular
 Ataxia Antibodies  >4 publications with ATG12 and Ataxia

Organs/Tissues associated with anti-ATG12 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >26 publications with ATG12 and Muscle
 Brain Antibodies  >25 publications with ATG12 and Brain
 Blood Antibodies  >18 publications with ATG12 and Blood
 Liver Antibodies  >13 publications with ATG12 and Liver
 Heart Antibodies  >10 publications with ATG12 and Heart
 Lung Antibodies  >9 publications with ATG12 and Lung
 Nerve Antibodies  >7 publications with ATG12 and Nerve
 Prostate Antibodies  >6 publications with ATG12 and Prostate
 Kidney Antibodies  >6 publications with ATG12 and Kidney
 Stomach Antibodies  >5 publications with ATG12 and Stomach
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