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KIAA0226 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153396

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Rubicon (KIAA0226), Polyclonal Antibody
Full Product Name
Rubicon Antibody
Product Gene Name
anti-KIAA0226 antibody
Product Synonym Names
Run domain Beclin-1 interacting and cysteine-rich containing protein; KIAA0226; KIAA0226
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Rubicon antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.
Preparation and Storage
Rubicon antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
Rubicon peptide (MBS154124) is used for blocking the activity of Rubicon antibody (MBS153396)
Molecular Weight
Predicted: 90; 101; 109 kDa

Observed: 91; 99 kDa
OMIM
gene 615705
Conjugate
Unconjugated
Immunogen
Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.
The immunogen is located within amino acids 240-290 of Rubicon.
Buffer
Rubicon antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-KIAA0226 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-p)
Application Notes for anti-KIAA0226 antibody
Optimal dilutions for each application to be determined by the researcher.

Western Blot (WB) of anti-KIAA0226 antibody

Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 μg/ml.
Rabbit Anti-KIAA0226 Polyclonal Antibody – Cat# MBS153396

Immunohistochemistry (IHC) of anti-KIAA0226 antibody

Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 μg/ml.
Rabbit Anti-KIAA0226 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS153396

Immunofluorescence (IF) of anti-KIAA0226 antibody

Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 μg/ml.
Rabbit Anti-KIAA0226 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153396

Other Notes

Small volumes of anti-KIAA0226 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KIAA0226 purchase

MBS153396 is an antibody targeting KIAA0226. Also known as Rubicon Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KIAA0226. It may not necessarily be applicable to this product.

NCBI GI #
530375960
NCBI GeneID
9711
NCBI Accession #
XP_005269431
UniProt Secondary Accession #
Q96CK5
UniProt Related Accession #
Q92622
GenBank Nucleotide #
XM_005269374.1
NCBI Official Full Name
run domain Beclin-1 interacting and cysteine-rich containing protein isoform X1
NCBI Official Synonym Full Names
KIAA0226
NCBI Official Symbol
KIAA0226
NCBI Official Synonym Symbols
SCAR15; RUBICON
UniProt Protein Name
Run domain Beclin-1 interacting and cysteine-rich containing protein
UniProt Synonym Protein Names
Beclin-1 associated RUN domain containing protein; Baron
Protein Family
Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
UniProt Gene Name
KIAA0226
UniProt Synonym Gene Names
Rubicon; Baron
UniProt Entry Name
RUBIC_HUMAN
NCBI Protein Information
run domain Beclin-1 interacting and cysteine-rich containing protein; baron; rundataxin; beclin-1 associated RUN domain containing protein; run domain Beclin-1 interacting and cystein-rich containing protein; RUN domain protein as Beclin 1-interacting and cysteine-rich containing; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein

NCBI Summary for KIAA0226

The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
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UniProt Comments for KIAA0226

rubicon: Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity. Forms a complex with BECN1, PIK3C3, PIK3R4 and UVRAG, but not with ATG14. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: Golgi apparatus; lysosome; late endosome; early endosome

Molecular Function: protein binding

Biological Process: negative regulation of autophagy; negative regulation of endocytosis; autophagy; endocytosis

Disease: Spinocerebellar Ataxia, Autosomal Recessive 15
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Research Articles on KIAA0226

1. This study demonistrated that KIAA0226 mutation impairs Rubicon endosomal localization

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-KIAA0226 antibody

 Disease Name  Pubmed Publications
 Ataxia Antibodies  >2 publications with KIAA0226 and Ataxia

Organs/Tissues associated with anti-KIAA0226 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >1 publications with KIAA0226 and Intestine
 Skin Antibodies  >1 publications with KIAA0226 and Skin
 Brain Antibodies  >1 publications with KIAA0226 and Brain
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