• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LIMA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153425

The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LIMA1, Polyclonal Antibody
Full Product Name
LIMA1 Antibody
Product Gene Name
Product Synonym Names
LIM domain and actin-binding protein 1; PP624; EPLIN; SREBP3 ; LIM domain and actin binding 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
LIMA1 antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this antibody will only detect the three largest isoforms.
Preparation and Storage
LIMA1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
LIMA1 peptide (MBS153860) is used for blocking the activity of LIMA1 antibody (MBS153425)
Molecular Weight
Predicted: 50; 66; 84 kDa

Observed: 65 kDa
OMIM
608364
3D Structure
ModBase 3D Structure for Q9UHB6
Conjugate
Unconjugated
Immunogen
LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1
Buffer
LIMA1 antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-LIMA1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-LIMA1 antibody
LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-LIMA1 antibody

Western blot analysis of LIMA1 in human colon tissue lysate with LIMA1 antibody at (A) 0.5 and (B) 1 μg/ml.
Rabbit Anti-LIMA1 Polyclonal Antibody – Cat# MBS153425

Immunohistochemistry (IHC) of anti-LIMA1 antibody

Immunohistochemistry of LIMA1 in human colon tissue with LIMA1 antibody at 5 μg/mL.
Rabbit Anti-LIMA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS153425

Immunofluorescence (IF) of anti-LIMA1 antibody

Immunofluorescence of LIMA1 in human colon tissue with LIMA1 antibody at 20 μg/mL.
Rabbit Anti-LIMA1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153425

Other Notes

Small volumes of anti-LIMA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIMA1 purchase

MBS153425 is an antibody targeting LIMA1. Also known as LIMA1 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LIMA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
LIM domain and actin-binding protein 1 isoform 1
NCBI Official Synonym Full Names
LIM domain and actin binding 1
NCBI Official Symbol
LIMA1
NCBI Official Synonym Symbols
EPLIN; SREBP3
UniProt Protein Name
LIM domain and actin-binding protein 1
UniProt Synonym Protein Names
Epithelial protein lost in neoplasm
UniProt Gene Name
LIMA1
UniProt Synonym Gene Names
EPLIN; SREBP3
UniProt Entry Name
LIMA1_HUMAN
NCBI Protein Information
LIM domain and actin-binding protein 1; 1110021C24Rik; epithelial protein lost in neoplasm beta; sterol regulatory element binding protein 3

NCBI Summary for LIMA1

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]
Show more

UniProt Comments for LIMA1

eplin: Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: focal adhesion; cytoplasm; plasma membrane; stress fiber; cleavage furrow; actin cytoskeleton

Molecular Function: actin monomer binding; actin filament binding; protein binding; zinc ion binding

Biological Process: actin filament bundle formation; ruffle organization and biogenesis; negative regulation of actin filament depolymerization
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000