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BTLA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153484

The B- and T-lymphocyte attenuator (BTLA) protein is a member of the immunoglobulin superfamily containing a single immunoglobulin (Ig) domain (1). Like other coihibitory receptors such as CTLA-4 and PD-1, BTLA functions as a receptor that relays inhibitory… More details

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Product Overview

Online Datasheet in PDF
Product Name
BTLA, Polyclonal Antibody
Full Product Name
BTLA Antibody
Product Gene Name
Product Synonym Names
BTLA Antibody : BTLA1; CD272; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; B and T lymphocyte associated
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
BTLA Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
BTLA antibody is human specific. At least two isoforms of BTLA are known to exist.
Preparation and Storage
BTLA antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
BTLA peptide (MBS154003) is used for blocking the activity of BTLA antibody (MBS153484)
Molecular Weight
Predicted: 32 kDa

Observed: 40kDa
OMIM
607925
3D Structure
ModBase 3D Structure for Q7Z6A9
Conjugate
Unconjugated
Immunogen
BTLA antibody was raised against a 17 amino acid peptide near the center of human BTLA.
Buffer
BTLA Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-BTLA antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-BTLA antibody
BTLA antibody can be used for detection of BTLA by Western blot at 1 - 2 mug/ml.

Western Blot (WB) of anti-BTLA antibody

Western blot analysis of BTLA in Jurkat cell lysate at (A) 1 and (B) 2 μg/ml.
Rabbit Anti-BTLA Polyclonal Antibody – Cat# MBS153484

Immunohistochemistry (IHC) of anti-BTLA antibody

Immunohistochemistry of BTLA in human small intestine tissue with BTLA antibody at 2.5 μg/mL.
Rabbit Anti-BTLA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS153484

Immunofluorescence (IF) of anti-BTLA antibody

Immunofluorescence of BTLA in human small intestine tissue with BTLA antibody at 20 μg/mL.
Rabbit Anti-BTLA Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153484

Other Notes

Small volumes of anti-BTLA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BTLA purchase

MBS153484 is an antibody targeting BTLA. Also known as BTLA Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BTLA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
B- and T-lymphocyte attenuator isoform 1
NCBI Official Synonym Full Names
B and T lymphocyte associated
NCBI Official Symbol
BTLA
NCBI Official Synonym Symbols
BTLA1; CD272
UniProt Protein Name
B- and T-lymphocyte attenuator
UniProt Synonym Protein Names
B- and T-lymphocyte-associated protein; CD_antigen: CD272
UniProt Gene Name
BTLA
UniProt Entry Name
BTLA_HUMAN
NCBI Protein Information
B- and T-lymphocyte attenuator; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein

NCBI Summary for BTLA

This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]
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UniProt Comments for BTLA

BTLA: Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: receptor activity

Biological Process: T cell costimulation; negative regulation of alpha-beta T cell proliferation; immune response-regulating cell surface receptor signaling pathway; negative regulation of B cell proliferation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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